PMID- 23623730
OWN - NLM
STAT- MEDLINE
DCOM- 20140130
LR  - 20130513
IS  - 1878-4186 (Electronic)
IS  - 0969-2126 (Linking)
VI  - 21
IP  - 5
DP  - 2013 May 7
TI  - An atomistic view of microtubule stabilization by GTP.
PG  - 833-43
LID - S0969-2126(13)00107-X [pii]
LID - 10.1016/j.str.2013.03.009 [doi]
AB  - A microtubule is a dynamic system formed of alphabeta-tubulins. The presence of 
      nonhydrolyzable guanosine-5'-triphosphate (GTP)/guanosine diphosphate (GDP) on 
      the beta-tubulins provokes microtubule polymerization/depolymerization. Despite the 
      large number of experimental studies of this dynamical process, its mechanism is 
      still unclear. To provide insights into this mechanism we studied the first 
      depolymerization steps of GDP/GTP-bound microtubules by normal-mode analysis with 
      the all-atom model. We also constructed a depolymerizing microtubule and compared 
      it to cryo-electron microscopy tomograms (cyro-ET). The results show that during 
      depolymerization, the protofilaments not only curve but twist to weaken their 
      lateral interactions. These interactions are stabilized by GTP, but not evenly. 
      Not all of the interface residues are of equal importance: five of them, 
      belonging to the H2-S3 loop, play a special role; acting as a lock whose key is 
      the gamma-phosphate of GTP. Sequence alignments of several tubulins confirm the 
      importance of these residues.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Quiniou, Eric
AU  - Quiniou E
AD  - Institut Curie, Centre de Recherche, U759, Bat. 112, Centre Universitaire, 91405 
      Orsay Cedex, France.
FAU - Guichard, Paul
AU  - Guichard P
FAU - Perahia, David
AU  - Perahia D
FAU - Marco, Sergio
AU  - Marco S
FAU - Mouawad, Liliane
AU  - Mouawad L
LA  - eng
PT  - Journal Article
DEP - 20130425
PL  - United States
TA  - Structure
JT  - Structure (London, England : 1993)
JID - 101087697
RN  - 0 (Tubulin)
RN  - 146-91-8 (Guanosine Diphosphate)
RN  - 86-01-1 (Guanosine Triphosphate)
SB  - IM
MH  - Cryoelectron Microscopy
MH  - Guanosine Diphosphate/chemistry/metabolism
MH  - Guanosine Triphosphate/chemistry/*metabolism
MH  - Kinetics
MH  - Microtubules/*chemistry/metabolism
MH  - Protein Conformation
MH  - Sequence Alignment
MH  - Tubulin/chemistry/metabolism
EDAT- 2013/04/30 06:00
MHDA- 2014/01/31 06:00
CRDT- 2013/04/30 06:00
PHST- 2012/10/29 00:00 [received]
PHST- 2013/02/08 00:00 [revised]
PHST- 2013/03/08 00:00 [accepted]
PHST- 2013/04/30 06:00 [entrez]
PHST- 2013/04/30 06:00 [pubmed]
PHST- 2014/01/31 06:00 [medline]
AID - S0969-2126(13)00107-X [pii]
AID - 10.1016/j.str.2013.03.009 [doi]
PST - ppublish
SO  - Structure. 2013 May 7;21(5):833-43. doi: 10.1016/j.str.2013.03.009. Epub 2013 Apr 
      25.