PMID- 23624044
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20130705
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 28
IP  - 4
DP  - 2013 Jun
TI  - Effect of recombinant adeno-associated virus mediated transforming growth 
      factor-beta1 on corneal allograft survival after high-risk penetrating 
      keratoplasty.
PG  - 164-9
LID - S0966-3274(13)00032-4 [pii]
LID - 10.1016/j.trim.2013.04.007 [doi]
AB  - Corneal transplantation is one of the most common and successful transplant 
      surgeries performed around the world. However, the high-risk corneal 
      transplantation remains a high level of corneal graft failure. Gene transfer of 
      immunomodulatory molecules is considered as one potential strategy in preventing 
      allograft rejection. It is worthy evaluating the effects of the immunemodulating 
      agent on corneal allograft rejection. The purpose of this paper is to investigate 
      the effects and mechanisms of recombinant adeno-associated virus mediated 
      transforming growth factor-beta1 (rAAV-TGF-beta1) on corneal allograft survival 
      using a high-risk rat model after penetrating keratoplasty (PKP). The mean 
      survival time (MST) of corneal grafts was observed and immuno-histochemical 
      staining of TGF-beta1 and Ox-62 was performed in the study. The MST showed 
      significant prolongation in the rAAV-TGF-beta1 group compared to the allograft 
      group. The rejection index (RI) at day 10 revealed was significantly greater in 
      the allograft group than that of the other two groups. Besides the increase of 
      TGF-beta1, the expression of Ox-62 decreasing in rAAV-TGF-beta1 transplanted 
      recipients was detected after transplantation. In short, treatment with 
      rAAV-TGF-beta1 prolongs corneal allograft survival and inhibits the Ox-62 
      expression in grafts after high-risk PKP.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zhou, Lianhong
AU  - Zhou L
AD  - Department of ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China. 
      zlh681102@yahoo.com.cn
FAU - Zhu, Xiangxiang
AU  - Zhu X
FAU - Tan, Jinquan
AU  - Tan J
FAU - Wang, Jiong
AU  - Wang J
FAU - Xing, Yiqiao
AU  - Xing Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130424
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (Antigens, Differentiation)
RN  - 0 (OX-62 antigen, rat)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/*biosynthesis
MH  - Cornea/metabolism
MH  - Dependovirus
MH  - Gene Transfer Techniques
MH  - *Genetic Therapy
MH  - Graft Rejection/*prevention & control
MH  - *Graft Survival
MH  - *Keratoplasty, Penetrating
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Survival Rate
MH  - Transforming Growth Factor beta1/*genetics
OTO - NOTNLM
OT  - Allograft survival
OT  - Dendritic cells
OT  - High-risk
OT  - Recombinant adeno-associated virus
OT  - Transforming growth factor-beta1
EDAT- 2013/04/30 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/04/30 06:00
PHST- 2012/11/30 00:00 [received]
PHST- 2013/04/10 00:00 [revised]
PHST- 2013/04/11 00:00 [accepted]
PHST- 2013/04/30 06:00 [entrez]
PHST- 2013/04/30 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - S0966-3274(13)00032-4 [pii]
AID - 10.1016/j.trim.2013.04.007 [doi]
PST - ppublish
SO  - Transpl Immunol. 2013 Jun;28(4):164-9. doi: 10.1016/j.trim.2013.04.007. Epub 2013 
      Apr 24.