PMID- 23624715
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20220317
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Print)
IS  - 1040-4651 (Linking)
VI  - 25
IP  - 4
DP  - 2013 Apr
TI  - The Arabidopsis B-BOX protein BBX25 interacts with HY5, negatively regulating 
      BBX22 expression to suppress seedling photomorphogenesis.
PG  - 1243-57
LID - 10.1105/tpc.113.109751 [doi]
AB  - ELONGATED HYPOCOTYL5 (HY5) is a basic domain/leucine zipper (bZIP) transcription 
      factor, central for the regulation of seedling photomorphogenesis. Here, we 
      identified a B-BOX (BBX)-containing protein, BBX25/SALT TOLERANCE HOMOLOG, as an 
      interacting partner of HY5, which has been previously found to physically 
      interact with CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). BBX25 physically interacts 
      with HY5 both in vitro and in vivo. By physiological and genetic approaches, we 
      showed that BBX25 is a negative regulator of seedling photomorphogenesis. BBX25 
      and its homolog BBX24 regulate deetiolation processes and hypocotyl shade 
      avoidance response in an additive manner. Moreover, genetic relationships of 
      bbx25 and bbx24 with hy5 and cop1 revealed that BBX25 and BBX24 additively 
      enhance COP1 and suppress HY5 functions. BBX25 accumulates in a light-dependent 
      manner and undergoes COP1-mediated degradation in dark and light conditions. 
      Furthermore, a protoplast cotransfection assay showed that BBX24 and BBX25 
      repress BBX22 expression by interfering with HY5 transcriptional activity. As HY5 
      binds to the BBX22 promoter and promotes its expression, our results identify a 
      direct mechanism through which the expression of BBX22 is regulated. We suggest 
      that BBX25 and BBX24 function as transcriptional corepressors, probably by 
      forming inactive heterodimers with HY5, downregulating BBX22 expression for the 
      fine-tuning of light-mediated seedling development.
FAU - Gangappa, Sreeramaiah N
AU  - Gangappa SN
AD  - Department of Biological and Environmental Sciences, Gothenburg University, 
      Gothenburg SE 40530, Sweden.
FAU - Crocco, Carlos D
AU  - Crocco CD
FAU - Johansson, Henrik
AU  - Johansson H
FAU - Datta, Sourav
AU  - Datta S
FAU - Hettiarachchi, Chamari
AU  - Hettiarachchi C
FAU - Holm, Magnus
AU  - Holm M
FAU - Botto, Javier F
AU  - Botto JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130426
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (BBX24 protein, Arabidopsis)
RN  - 0 (BBX25 protein, Arabidopsis)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (HY5 protein, Arabidopsis)
RN  - 0 (LZF1 protein, Arabidopsis)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
CIN - Plant Cell. 2013 Apr;25(4):1192-5. PMID: 23847786
MH  - Arabidopsis/*genetics/growth & development/radiation effects
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Basic-Leucine Zipper Transcription Factors/*genetics/metabolism
MH  - Gene Expression Regulation, Developmental/radiation effects
MH  - Gene Expression Regulation, Plant/radiation effects
MH  - Hypocotyl/genetics/growth & development/radiation effects
MH  - Immunoblotting
MH  - Light
MH  - Luminescent Proteins/genetics/metabolism
MH  - Microscopy, Fluorescence
MH  - Models, Genetic
MH  - Nuclear Proteins/*genetics/metabolism
MH  - Protein Binding
MH  - Protoplasts/metabolism
MH  - Repressor Proteins/*genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seedlings/*genetics/growth & development/radiation effects
MH  - Transcription Factors/*genetics/metabolism
MH  - Two-Hybrid System Techniques
MH  - Ubiquitin-Protein Ligases
PMC - PMC3663265
EDAT- 2013/04/30 06:00
MHDA- 2014/01/01 06:00
PMCR- 2014/04/01
CRDT- 2013/04/30 06:00
PHST- 2013/04/30 06:00 [entrez]
PHST- 2013/04/30 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - tpc.113.109751 [pii]
AID - 109751 [pii]
AID - 10.1105/tpc.113.109751 [doi]
PST - ppublish
SO  - Plant Cell. 2013 Apr;25(4):1243-57. doi: 10.1105/tpc.113.109751. Epub 2013 Apr 
      26.