PMID- 23625978
OWN - NLM
STAT- MEDLINE
DCOM- 20140429
LR  - 20220129
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 73
IP  - 4
DP  - 2014 Apr
TI  - Expression of aberrant HLA-B27 molecules is dependent on B27 dosage and peptide 
      supply.
PG  - 763-70
LID - 10.1136/annrheumdis-2012-203080 [doi]
AB  - OBJECTIVES: Cellular expression of non-classical forms of human leukocyte antigen 
      (HLA)-B27 (NC-B27) may be involved in spondyloarthritis (SpA) pathogenesis. We 
      used a novel B27-specific monoclonal antibody, HD6, to ask if B27 transgenic (TG) 
      rat splenocytes express these NC-B27 molecules. We also investigated whether 
      B27-binding peptides could affect the expression and functional immune 
      recognition of HD6-reactive B27 molecules. METHODS: Splenocytes from B27-TG, 
      B7-TG and non-transgenic rats, and HLA-B27+ cell lines were stained with 
      monoclonal antibodies recognising classical (ME-1, HLA-ABC-m1) and non-classical 
      (HD6, HC10) B27. Cells were further cultured in the presence of HLA-B27-binding 
      peptides, or subjected to brief low pH treatment prior to mAb staining and/or 
      immunoprecipitation or co-culture with KIR3DL2-CD3epsilon-expressing Jurkat reporter 
      cells. RESULTS: HD6-reactive molecules were detected in the majority of adult 
      B27-TG rat splenocyte cell subsets, increasing with age and concomitant increased 
      B27 expression. HD6 staining was inhibited by incubation with B27-binding 
      peptides and induced by low pH treatment. HD6 staining correlated with 
      KIR3DL2-CD3epsilon-expressing Jurkat reporter cell activity. Thus, IL-2 production was 
      decreased when B27-expressing antigen-presenting cells were preincubated with 
      B27-binding peptides, but increased following pretreatment with low pH buffer. 
      CONCLUSIONS: Surface expression of HD6-reactive B27 molecules on B27-TG rat 
      splenocytes is consistent with a pathogenic role for NC-B27 in SpA. Interaction 
      of NC-B27 with innate immune receptors could be critical in SpA pathogenesis, and 
      we show that this may be influenced by the availability and composition of the 
      B27-binding peptide pool.
FAU - McHugh, Kirsty
AU  - McHugh K
AD  - Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Science, 
      University of Oxford, , Oxford, UK.
FAU - Rysnik, Oliwia
AU  - Rysnik O
FAU - Kollnberger, Simon
AU  - Kollnberger S
FAU - Shaw, Jacqueline
AU  - Shaw J
FAU - Utriainen, Lotta
AU  - Utriainen L
FAU - Al-Mossawi, Mohammad Hussein
AU  - Al-Mossawi MH
FAU - Payeli, Sravan
AU  - Payeli S
FAU - Belaunzaran, Osiris Marroquin
AU  - Belaunzaran OM
FAU - Milling, Simon
AU  - Milling S
FAU - Renner, Christoph
AU  - Renner C
FAU - Bowness, Paul
AU  - Bowness P
LA  - eng
GR  - 19380/VAC_/Versus Arthritis/United Kingdom
GR  - 18599/ARC_/Arthritis Research UK/United Kingdom
GR  - 19611/VAC_/Versus Arthritis/United Kingdom
GR  - 19807/VAC_/Versus Arthritis/United Kingdom
GR  - 19611/ARC_/Arthritis Research UK/United Kingdom
GR  - 19807/ARC_/Arthritis Research UK/United Kingdom
GR  - 19853/ARC_/Arthritis Research UK/United Kingdom
GR  - 19853/VAC_/Versus Arthritis/United Kingdom
GR  - 18599/VAC_/Versus Arthritis/United Kingdom
GR  - 19380/ARC_/Arthritis Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130426
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (KIR3DL2 protein, human)
RN  - 0 (Peptides)
RN  - 0 (Receptors, KIR3DL2)
SB  - IM
EIN - Ann Rheum Dis. 2016 Jun;75(6):1254. PMID: 27166138
MH  - Aging/immunology
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antigen-Presenting Cells/immunology
MH  - Cell Line
MH  - Coculture Techniques
MH  - *Gene Dosage
MH  - HLA-B27 Antigen/genetics/*metabolism
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Jurkat Cells
MH  - Peptides/*metabolism
MH  - Rats
MH  - Rats, Transgenic
MH  - Receptors, KIR3DL2/metabolism
MH  - Spleen/cytology/*immunology
MH  - Spondylarthritis/immunology
EDAT- 2013/04/30 06:00
MHDA- 2014/04/30 06:00
CRDT- 2013/04/30 06:00
PHST- 2013/04/30 06:00 [entrez]
PHST- 2013/04/30 06:00 [pubmed]
PHST- 2014/04/30 06:00 [medline]
AID - annrheumdis-2012-203080 [pii]
AID - 10.1136/annrheumdis-2012-203080 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2014 Apr;73(4):763-70. doi: 10.1136/annrheumdis-2012-203080. Epub 
      2013 Apr 26.