PMID- 23627062
OWN - NLM
STAT- MEDLINE
DCOM- 20130612
LR  - 20190923
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 9
IP  - 2
DP  - 2013 Feb
TI  - Preparation of functional magnetic nanoparticles mediated with PEG-4000 and 
      application in Pseudomonas aeruginosa rapid detection.
PG  - 312-7
AB  - A rapid detection method of Pseudomonas aeruginosa based on magnetic separation 
      and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) 
      were prepared by solvothermal method with PEG-4000 as a surfactant, and then were 
      modified. The prepared MNPs present a uniform morphology and good dispersion. The 
      sizes of MNPs can be controlled by adjusting the dosage of FeCl3 x 6H2O. The 
      obtained particles were characterized with Scanning electron microscope (SEM), 
      Transmission electronic microscopy (TEM) and Fourier transform infrared (FTIR). 
      The biotin-dUTP-labeled DNA fragments of gyrB gene were amplified by polymerase 
      chain reaction (PCR), and Pseudomonas aeruginosa was successfully detected with 
      detection limit as low as 7.5 fM of gyrB fragments.
FAU - Tang, Yongjun
AU  - Tang Y
AD  - State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, 
      China.
FAU - Li, Zhiyang
AU  - Li Z
FAU - He, Nongyue
AU  - He N
FAU - Zhang, Liming
AU  - Zhang L
FAU - Ma, Chao
AU  - Ma C
FAU - Li, Xiaolong
AU  - Li X
FAU - Li, Chuanyan
AU  - Li C
FAU - Wang, Zhifei
AU  - Wang Z
FAU - Deng, Yan
AU  - Deng Y
FAU - He, Lei
AU  - He L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
RN  - 0 (Magnetite Nanoparticles)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Electrophoresis, Agar Gel
MH  - Luminescent Measurements
MH  - Magnetite Nanoparticles/*chemistry/ultrastructure
MH  - Polyethylene Glycols/*chemistry
MH  - Pseudomonas aeruginosa/*isolation & purification/ultrastructure
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2013/05/01 06:00
MHDA- 2013/06/13 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2013/06/13 06:00 [medline]
AID - 10.1166/jbn.2013.1493 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2013 Feb;9(2):312-7. doi: 10.1166/jbn.2013.1493.