PMID- 23627371
OWN - NLM
STAT- MEDLINE
DCOM- 20130913
LR  - 20191210
IS  - 1744-5205 (Electronic)
IS  - 0882-0538 (Linking)
VI  - 28
IP  - 4
DP  - 2013 Jul
TI  - Evaluation of direct immunofluorescence assay and cytological examination in 
      comparison to polymerase chain reaction of conjunctival swabs in patients with 
      adult inclusion conjunctivitis.
PG  - 216-23
LID - 10.3109/08820538.2012.760623 [doi]
AB  - PURPOSE: To evaluate PCR, direct immunofluorescence assay (DIA) and cytological 
      test of conjunctival swabs for the diagnosis of adult follicular conjunctivitis 
      (AFC). METHODS: Eighty-three adult patients with chronic conjunctivitis and 
      sixteen healthy individuals were included. Conjunctival scrapings underwent PCR, 
      DIA and cytological analysis. Exams were repeated two weeks after treatment 
      application. Sensitivity, specificity and agreement rate with PCR of DIA and 
      Cytology were evaluated and correlated with clinical symptoms/signs. RESULTS: 
      Cytology test was more sensitive than DIA and presented an acceptable agreement 
      with PCR (K=0.44) in treatment-naive patients, concerning especially the 
      combination of both conventional exams (K = 0.77). Inferior diagnostic 
      performance of was detected post-treatment, considering the combination as well 
      (K=0.40). Negative post-treatment PCR correlated well with significant relief of 
      symptoms/signs. CONCLUSION: Combination of Cytology and DIA seems to be a useful 
      diagnostic option for treatment naive AFC patients. However, PCR remains the most 
      reliable test for post-treatment evaluation.
FAU - Malamos, Panagiotis
AU  - Malamos P
AD  - Department of Ophthalmology, G. Gennimatas General Hospital, Athens, Greece.
FAU - Georgalas, Ilias
AU  - Georgalas I
FAU - Rallis, Konstantinos
AU  - Rallis K
FAU - Andrianopoulos, Konstantinos
AU  - Andrianopoulos K
FAU - Konstantoulakis, Panteleimon
AU  - Konstantoulakis P
FAU - Georgopoulos, Gerasimos
AU  - Georgopoulos G
FAU - Theodosiadis, Panagiotis
AU  - Theodosiadis P
FAU - Markomichelakis, Nikos N
AU  - Markomichelakis NN
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20130429
PL  - England
TA  - Semin Ophthalmol
JT  - Seminars in ophthalmology
JID - 8610759
SB  - IM
MH  - Chronic Disease
MH  - Conjunctiva/*pathology
MH  - Conjunctivitis, Inclusion/*diagnosis
MH  - *Cytological Techniques
MH  - False Positive Reactions
MH  - *Fluorescent Antibody Technique, Direct
MH  - Humans
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - *Real-Time Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Surveys and Questionnaires
EDAT- 2013/05/01 06:00
MHDA- 2013/09/14 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2013/09/14 06:00 [medline]
AID - 10.3109/08820538.2012.760623 [doi]
PST - ppublish
SO  - Semin Ophthalmol. 2013 Jul;28(4):216-23. doi: 10.3109/08820538.2012.760623. Epub 
      2013 Apr 29.