PMID- 23627845
OWN - NLM
STAT- MEDLINE
DCOM- 20140616
LR  - 20131104
IS  - 1502-3850 (Electronic)
IS  - 0001-6357 (Linking)
VI  - 71
IP  - 6
DP  - 2013 Nov
TI  - Early root surface colonization by human periodontal ligament fibroblasts 
      following treatment with different biomaterials.
PG  - 1579-87
LID - 10.3109/00016357.2013.777115 [doi]
AB  - OBJECTIVES: The present in-vitro study examined the effects of different 
      biomaterials on early root surface colonization by human periodontal ligament 
      (PDL) fibroblasts using confocal-laser-scanning-microscopy (CLSM). MATERIALS AND 
      METHODS: Fifteen periodontally-diseased teeth were extracted, treated with 
      scaling/root planing and longitudinally cut to obtain 30 root fragments. 
      Fragments were treated either with 24% EDTA following application of enamel 
      matrix derivative (EMD), 24% EDTA or EMD only, nanocrystalline hydroxyapatite 
      (NHA) paste or oily calcium hydroxide suspension (OCHS) for 1 h each. The 
      analogue untreated root specimens served as controls. Root fragments were 
      incubated with human PDL fibroblasts and cellular proliferation and morphology 
      were evaluated after 1, 3, 5 and 8 days using CLSM-visualization and image 
      recognition software. RESULTS: The rate of cellular proliferation was different 
      among treatment modalities examined (p = 0.019). Except treatment with NHA paste 
      all treatment modalities improved cellular proliferation on root surfaces at all 
      different points of time compared with the control specimens. A significant 
      difference between treatment modalities was observed between EMD and NHA paste (p 
      = 0.008). No synergistic effect could be demonstrated comparing root surface 
      conditioning with 24% EDTA and EMD application compared to 24% EDTA or EMD 
      application only. CONCLUSION: The present results suggest that initial root 
      surface colonization by PDL fibroblasts may be enhanced by root surface 
      conditioning with 24% EDTA and application of EMD, application of 24% EDTA or EMD 
      alone and OCHS. The addition of 24% EDTA for root surface conditioning prior to 
      EMD application provided no synergistic effects in terms of early root surface 
      colonization by PDL fibroblasts.
FAU - Kasaj, Adrian
AU  - Kasaj A
AD  - Department of Operative Dentistry and Periodontology.
FAU - Klein, Marcus O
AU  - Klein MO
FAU - Dupont, Julia
AU  - Dupont J
FAU - Willershausen, Brita
AU  - Willershausen B
FAU - Krahn, Ulrike
AU  - Krahn U
FAU - Gotz, Hermann
AU  - Gotz H
FAU - Zeiler, Johannes
AU  - Zeiler J
FAU - Brullmann, Dan
AU  - Brullmann D
FAU - Duschner, Heinz
AU  - Duschner H
LA  - eng
PT  - Journal Article
DEP - 20130429
PL  - Sweden
TA  - Acta Odontol Scand
JT  - Acta odontologica Scandinavica
JID - 0370344
RN  - 0 (Biocompatible Materials)
SB  - IM
MH  - *Biocompatible Materials
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Microscopy, Confocal
MH  - Periodontal Ligament/*cytology
MH  - Tooth Root/*microbiology
EDAT- 2013/05/01 06:00
MHDA- 2014/06/17 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2014/06/17 06:00 [medline]
AID - 10.3109/00016357.2013.777115 [doi]
PST - ppublish
SO  - Acta Odontol Scand. 2013 Nov;71(6):1579-87. doi: 10.3109/00016357.2013.777115. 
      Epub 2013 Apr 29.