PMID- 23628016
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20181023
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 21
IP  - 2
DP  - 2013 Apr
TI  - [Effects of eEF1A1 re-expression on proliferation and apoptosis of Jurkat cells 
      with knocked down eEF1A1 gene and its mechanisms].
PG  - 279-84
LID - 10.7534/j.issn.1009-2137.2013.02.004 [doi]
AB  - This study was aimed to explore the effects of expressing eukaryotic elongation 
      factor 1A1 (eEF1A1) on proliferation and apoptosis in human acute T lymphocytic 
      leukemia (T-ALL) cell line Jurkat with knocked down eEF1A1 gene and its 
      mechanisms. eEF1A1-expressing lentivirus (LV) was constructed and used to 
      transfect the Jurkat cells with knocked down eEF1A1 gene. Then, the expressions 
      of eEF1A1 mRNA and protein were detected by real time PCR(RT-PCR) and Western 
      blot respectively.Cell proliferation, apoptosis and cycle were detected by MTT 
      method, Annexin V-APC labeling and DNA ploidy analysis respectively. The related 
      protein expressions of phosphatidylinositol-3-kinase (PI3K)/serine/threonine 
      kinase (Akt) signaling pathway were detected by Western blot. The results 
      indicated that eEF1A1 mRNA and protein expressions of Jurkat cells with knocked 
      down eEF1A1 gene were re-established by constructing eEF1A1-expression LV. 
      Compared with negative control group (transfected with negative control LV and 
      eEF1A1-shRNA LV), cell proliferation in eEF1A1 expression group was significantly 
      enhanced, cell apoptosis was remarkably inhibited, percentage of cells in G0/G1 
      phase was significantly reduced alone with increased percentage of cells in S and 
      G2/M phase, and the expression levels of p-Akt (Ser 473), nuclear factor kappa B 
      (NF-kappaB), p-NF-kappaB (Ser 468), mammalian target of rapamycin (mTOR) and p-mTOR (Ser 
      2448) protein significantly increased. It is concluded that eEF1A1 may have a 
      carcinogenic effect in T-ALL cells. eEF1A1 expression has noticeable effects on 
      the proliferation enhancement and apoptosis inhibition of Jurkat cells, which may 
      be mediated by the up-regulation of PI3K/Akt/NF-kappaB and PI3K/Akt/ mTOR signaling 
      pathway.
FAU - Huang, Yi
AU  - Huang Y
AD  - Fujian Medical University, Fuzhou, Fujian Province, China. Hyi8070@126.com
FAU - Hu, Jian-Da
AU  - Hu JD
FAU - Wu, Yan-An
AU  - Wu YA
FAU - Zheng, Jing
AU  - Zheng J
FAU - Qi, Yuan-Lin
AU  - Qi YL
FAU - Chen, Ying-Yu
AU  - Chen YY
FAU - Huang, Xiao-Li
AU  - Huang XL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
RN  - 0 (EEF1A1 protein, human)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - *Apoptosis
MH  - *Cell Proliferation
MH  - Gene Expression
MH  - Humans
MH  - Jurkat Cells
MH  - Peptide Elongation Factor 1/*genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction
EDAT- 2013/05/01 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - 1009-2137(2013)02-0279-06 [pii]
AID - 10.7534/j.issn.1009-2137.2013.02.004 [doi]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2013 Apr;21(2):279-84. doi: 
      10.7534/j.issn.1009-2137.2013.02.004.