PMID- 23628396
OWN - NLM
STAT- MEDLINE
DCOM- 20140124
LR  - 20130705
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 74
IP  - 8
DP  - 2013 Aug
TI  - Anti-HLA-A, -B, -DR, -DQB1 and -DQA1 antibodies reactive epitope determination 
      with HLAMatchmaker in multipare awaiting list for heart transplant.
PG  - 937-41
LID - S0198-8859(13)00119-5 [pii]
LID - 10.1016/j.humimm.2013.04.033 [doi]
AB  - Human leukocyte antigen (HLA) antibodies represent a significant risk factor for 
      transplant failure. It is very important to characterize anti-HLA antibodies as 
      epitopes rather than antigens so that this knowledge can be applied clinically. 
      The aim of the study was to investigate the extra reactivity patterns in 
      sensitized multipare. Here, we have used the HLAMatchmaker program, a theoretical 
      algorithm, to explain these unexpected antibody reactivity patterns in multipare 
      awaiting for heart transplant. The patient was sensitized during pregnancy by 
      alleles HLA-A(*)24:02, HLA-DRB1(*)07:01, HLA-DRB4(*)01:01, DQB1(*)02:02 and 
      DQA1(*)02:01 mismatches with development of respective antibodies. However, the 
      patient' sera were shown an unexpected reactivity not directed toward HLA 
      mismatches of daughters: A( *)23:01, A(*)24:03 and B(*)15:12 for class I and 
      DRB4(*)01:03, DRB1(*)09:02, DRB1(*)09:01, DQB1(*)03:01, DQB1(*)03:03, 
      DQB1(*)03:02, DQB1(*)04:02, DQB1(*)04:01 and DQB1(*)02:01 for class II. By 
      HLAMatchmaker analysis we found that these antibodies reacted with eplet shared 
      by antigens in single allele Luminex panels. These eplets were: 62EE, 66GKH, 
      70KAH, 71HS, 127K, 113YH, 144KR, 150AAH, 151AHV, 163TG and 167DG for class I and 
      4Q, 74RRAE, 71RRA, 98KN, 120N, and 135G, 25FT, 34HE, 41ER, 47EK2, 48LF for class 
      II. Thus, HLAMatchmaker software together with to solid phase techniques could 
      open new horizons for a more precise characterization of the HLA-antibodies.
CI  - Copyright (c) 2013. Published by Elsevier Inc.
FAU - Resse, Marianna
AU  - Resse M
AD  - U.O.C. Immunohematology, Transfusion Medicine and Transplant Immunology (SIMT), 
      Regional Reference Laboratory of Transplant Immunology (LIT), Azienda 
      Universitaria Policlinico (AOU), Second University of Naples, Italy. 
      marianna.resse@unina2.it
FAU - Paolillo, Rossella
AU  - Paolillo R
FAU - Casamassimi, Amelia
AU  - Casamassimi A
FAU - Cavalca, Francesco
AU  - Cavalca F
FAU - Fiorito, Carmela
AU  - Fiorito C
FAU - Maiello, Ciro
AU  - Maiello C
FAU - Napoli, Claudio
AU  - Napoli C
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20130427
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antibodies)
RN  - 0 (Epitopes)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Antibodies/*immunology
MH  - Antibody Specificity/genetics/immunology
MH  - Epitopes/*immunology
MH  - Female
MH  - HLA Antigens/genetics/*immunology
MH  - *Heart Transplantation
MH  - Histocompatibility/genetics/immunology
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Middle Aged
OTO - NOTNLM
OT  - CDC
OT  - CDR
OT  - ELISA
OT  - HLA
OT  - MFI
OT  - PCR-SSP
OT  - PRA
OT  - SA
OT  - complement-dependent cytotoxicity crossmatch
OT  - complementary determining region
OT  - enzyme-linked immunosorbent assay
OT  - human leukocyte antigen
OT  - mean fluorescence intensity units
OT  - panel reactive antibody
OT  - polymerase chain reaction-sequence-specific primer
OT  - single antigens
EDAT- 2013/05/01 06:00
MHDA- 2014/01/25 06:00
CRDT- 2013/05/01 06:00
PHST- 2012/10/24 00:00 [received]
PHST- 2013/03/05 00:00 [revised]
PHST- 2013/04/10 00:00 [accepted]
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2014/01/25 06:00 [medline]
AID - S0198-8859(13)00119-5 [pii]
AID - 10.1016/j.humimm.2013.04.033 [doi]
PST - ppublish
SO  - Hum Immunol. 2013 Aug;74(8):937-41. doi: 10.1016/j.humimm.2013.04.033. Epub 2013 
      Apr 27.