PMID- 23628658
OWN - NLM
STAT- MEDLINE
DCOM- 20140203
LR  - 20211021
IS  - 1976-555X (Electronic)
IS  - 1229-845X (Print)
IS  - 1229-845X (Linking)
VI  - 14
IP  - 2
DP  - 2013
TI  - Genetic diversity of porcine reproductive and respiratory syndrome virus in 
      Korea.
PG  - 115-24
AB  - The high genetic diversity of porcine reproductive and respiratory syndrome virus 
      (PRRSV) has been an obstacle to developing an effective vaccine for porcine 
      reproductive and respiratory syndrome (PRRS). This study was performed to assess 
      the degree of genetic diversity among PRRSVs from Korean pig farms where wasting 
      and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms 
      were tested for the presence of PRRSV using reverse transcription PCR protocol. A 
      total of 117 farms were positive for type 1 PRRSV while 198 farms were positive 
      for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were 
      analyzed and compared to those of various published PRRSV isolates obtained 
      worldwide. Sequence identity of the ORF 5 in the isolates was 81.6 100% for type 
      1 viruses and 81.4 100% for type 2 viruses. Phylogenetic analysis of the ORF 5 
      sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into 
      three and four clusters, respectively. The analyzed isolates were distributed 
      throughout the clusters independent of the isolation year or geographical origin. 
      In conclusion, our results indicated that the genetic diversity of PRRSVs from 
      Korean pig farms is high and has been increasing over time.
FAU - Choi, Eun-Jin
AU  - Choi EJ
AD  - Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural 
      Affairs, Anyang 430-757, Korea.
FAU - Lee, Chang-Hee
AU  - Lee CH
FAU - Song, Jae-Young
AU  - Song JY
FAU - Song, Hee-Jong
AU  - Song HJ
FAU - Park, Choi-Kyu
AU  - Park CK
FAU - Kim, Byounghan
AU  - Kim B
FAU - Shin, Yeun-Kyung
AU  - Shin YK
LA  - eng
PT  - Journal Article
DEP - 20130430
PL  - Korea (South)
TA  - J Vet Sci
JT  - Journal of veterinary science
JID - 100964185
SB  - IM
MH  - Animal Husbandry
MH  - Animals
MH  - *Genes, Viral
MH  - *Genetic Variation
MH  - Lung/virology
MH  - Lymph Nodes/virology
MH  - *Open Reading Frames
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/chemistry/classification/*genetics/isolation & purification
MH  - Republic of Korea
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Analysis, DNA/veterinary
MH  - Sequence Analysis, Protein/veterinary
MH  - Swine
PMC - PMC3694182
OTO - NOTNLM
OT  - Korea
OT  - open reading frame 5
OT  - phylogenetic analysis
OT  - porcine reproductive and respiratory syndrome virus
EDAT- 2013/05/01 06:00
MHDA- 2014/02/04 06:00
PMCR- 2013/06/01
CRDT- 2013/05/01 06:00
PHST- 2011/08/19 00:00 [received]
PHST- 2012/04/04 00:00 [accepted]
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2014/02/04 06:00 [medline]
PHST- 2013/06/01 00:00 [pmc-release]
AID - jvs.2013.022 [pii]
AID - 10.4142/jvs.2013.14.2.115 [doi]
PST - ppublish
SO  - J Vet Sci. 2013;14(2):115-24. doi: 10.4142/jvs.2013.14.2.115. Epub 2013 Apr 30.