PMID- 23628856
OWN - NLM
STAT- MEDLINE
DCOM- 20140212
LR  - 20160307
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 43
IP  - 8
DP  - 2013 Aug
TI  - FISH identification of Helicoverpa armigera and Mamestra brassicae chromosomes by 
      BAC and fosmid probes.
PG  - 644-53
LID - S0965-1748(13)00070-2 [pii]
LID - 10.1016/j.ibmb.2013.04.003 [doi]
AB  - Since the Bombyx mori genome sequence was published, conserved synteny between B. 
      mori and some other lepidopteran species has been revealed by either FISH 
      (fluorescence in situ hybridization) with BAC (bacterial artificial chromosome) 
      probes or linkage analysis. However, no species belonging to the Noctuidae, the 
      largest lepidopteran family which includes serious polyphagous pests, has been 
      analyzed so far with respect to genome-wide conserved synteny and gene order. For 
      that purpose, we selected the noctuid species Helicoverpa armigera and Mamestra 
      brassicae, both with n = 31 chromosomes. Gene-defined fosmid clones from M. 
      brassicae and BAC clones from a closely related species of H. armigera, Heliothis 
      virescens, were used for a FISH analysis on pachytene chromosomes. We recognized 
      all H. armigera chromosomes from specific cross-hybridization signals of 146 BAC 
      probes. With 100 fosmid clones we identified and characterized all 31 bivalents 
      of M. brassicae. Synteny and gene order were well conserved between the two 
      noctuid species. The comparison with the model species B. mori (n = 28) showed 
      the same phenomenon for 25 of the 28 chromosomes. Three chromosomes (#11, #23 and 
      #24) had two counterparts each in H. armigera and M. brassicae. Since n = 31 is 
      the modal chromosome number in Lepidoptera, the noctuid chromosomes probably 
      represent an ancestral genome organization of Lepidoptera. This is the first 
      identification of a full karyotype in Lepidoptera by means of BAC 
      cross-hybridization between species. The technique shows the potential to expand 
      the range of analyzed species efficiently.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Sahara, Ken
AU  - Sahara K
AD  - Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka 020-8550, Japan. 
      sahara@iwate-u.ac.jp
FAU - Yoshido, Atsuo
AU  - Yoshido A
FAU - Shibata, Fukashi
AU  - Shibata F
FAU - Fujikawa-Kojima, Noriko
AU  - Fujikawa-Kojima N
FAU - Okabe, Takuya
AU  - Okabe T
FAU - Tanaka-Okuyama, Makiko
AU  - Tanaka-Okuyama M
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130428
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
SB  - IM
EIN - Insect Biochem Mol Biol. 2015 Nov;66:145-6
MH  - Animals
MH  - *Chromosome Mapping
MH  - Chromosomes, Artificial, Bacterial
MH  - *Chromosomes, Insect
MH  - Genome, Insect
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Probe Techniques
MH  - Moths/*genetics
OTO - NOTNLM
OT  - BAC-FISH
OT  - Gene mapping
OT  - Insect
OT  - Lepidoptera
OT  - Pachytene bivalents
EDAT- 2013/05/01 06:00
MHDA- 2014/02/13 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/02/13 00:00 [received]
PHST- 2013/04/09 00:00 [revised]
PHST- 2013/04/15 00:00 [accepted]
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2014/02/13 06:00 [medline]
AID - S0965-1748(13)00070-2 [pii]
AID - 10.1016/j.ibmb.2013.04.003 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2013 Aug;43(8):644-53. doi: 10.1016/j.ibmb.2013.04.003. 
      Epub 2013 Apr 28.