PMID- 23629843
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20130611
IS  - 1099-0801 (Electronic)
IS  - 0269-3879 (Linking)
VI  - 27
IP  - 7
DP  - 2013 Jul
TI  - Isolation of volatiles from Nigella sativa seeds using microwave-assisted 
      extraction: effect of whole extracts on canine and murine CYP1A.
PG  - 938-45
LID - 10.1002/bmc.2887 [doi]
AB  - The volatile components of Nigella sativa seeds were isolated using 
      microwave-assisted extraction (MAE) and identified using gas chromatography. 
      Further investigations were carried out to demonstrate the effects of whole 
      extracts on canine (dog) and murine (rat) cytochrome P450 1A (CYP1A). The optimal 
      extraction conditions of MAE were as follows: 25 mL of water, medium level of 
      microwave oven power and 10 min of extraction time. A total of 32 compounds were 
      identified under the conditions using GC-FID and GC-MS. Thymoquinone (38.23%), 
      p-cymene (28.61%), 4-isopropyl-9-methoxy-1-methyl-1-cyclohexene (5.74%), 
      longifolene (5.33%), alpha-thujene (3.88) and carvacol (2.31%) were the main 
      compounds emitted from N. sativa seeds. Various extracts including pure 
      compounds, essential oil, nonpolar partition, relatively high-polar/nonpolar 
      partition, and polar partition extracts effectively inhibited the reaction of 
      ethoxyresorufin O-de-ethylation, which is specified for CYP1A activity both in 
      dog and rat. This in vitro data should be heeded as a signal of possible in vivo 
      interactions. The use of human liver preparations would considerably strengthen 
      the practical impact of the data generated from this study.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Liu, Xue
AU  - Liu X
AD  - The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, 
      School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu 
      Avenue, Wuxi, 214122, China.
FAU - Park, Jong-Hyouk
AU  - Park JH
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
FAU - Assayed, M E
AU  - Assayed ME
FAU - Shimoda, Minoru
AU  - Shimoda M
FAU - Shim, Jae-Han
AU  - Shim JH
LA  - eng
PT  - Journal Article
DEP - 20130430
PL  - England
TA  - Biomed Chromatogr
JT  - Biomedical chromatography : BMC
JID - 8610241
RN  - 0 (Organic Chemicals)
RN  - 0 (Oxazines)
RN  - 0 (Plant Extracts)
RN  - 5725-91-7 (ethoxyresorufin)
RN  - EC 1.14.14.1 (Aryl Hydrocarbon Hydroxylases)
SB  - IM
MH  - Animals
MH  - Aryl Hydrocarbon Hydroxylases/*drug effects/metabolism
MH  - Chemical Fractionation/*methods
MH  - Dogs
MH  - Kinetics
MH  - Microsomes/metabolism
MH  - Microwaves
MH  - Nigella sativa/*chemistry
MH  - Organic Chemicals/chemistry/*isolation & purification/pharmacology
MH  - Oxazines/metabolism
MH  - Plant Extracts/chemistry/*isolation & purification/pharmacology
MH  - Rats
MH  - Seeds/*chemistry
EDAT- 2013/05/01 06:00
MHDA- 2014/04/08 06:00
CRDT- 2013/05/01 06:00
PHST- 2012/11/22 00:00 [received]
PHST- 2013/01/14 00:00 [revised]
PHST- 2013/01/28 00:00 [accepted]
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
AID - 10.1002/bmc.2887 [doi]
PST - ppublish
SO  - Biomed Chromatogr. 2013 Jul;27(7):938-45. doi: 10.1002/bmc.2887. Epub 2013 Apr 
      30.