PMID- 23629861
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20231213
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 27
IP  - 8
DP  - 2013 Aug
TI  - Basic evidence for epidermal H2O2/ONOO(-)-mediated oxidation/nitration in 
      segmental vitiligo is supported by repigmentation of skin and eyelashes after 
      reduction of epidermal H2O2 with topical NB-UVB-activated pseudocatalase PC-KUS.
PG  - 3113-22
LID - 10.1096/fj.12-226779 [doi]
AB  - Nonsegmental vitiligo (NSV) is characterized by loss of inherited skin color. The 
      cause of the disease is still unknown despite accumulating in vivo and in vitro 
      evidence of massive epidermal oxidative stress via H2O2 and peroxynitrite 
      (ONOO(-)) in affected individuals. The most favored hypothesis is based on 
      autoimmune mechanisms. Strictly segmental vitiligo (SSV) with dermatomal 
      distribution is a rare entity, often associated with stable outcome. Recently, it 
      was documented that this form can be associated with NSV (mixed vitiligo). We 
      here asked the question whether ROS and possibly ONOO(-) could be players in the 
      pathogenesis of SSV. Our in situ results demonstrate for the first time epidermal 
      biopterin accumulation together with significantly decreased epidermal catalase, 
      thioredoxin/thioreoxin reductase, and MSRA/MSRB expression. Moreover, we show 
      epidermal ONOO(-) accumulation. In vivo FT-Raman spectroscopy reveals the 
      presence of H2O2, methionine sulfoxide, and tryptophan metabolites; i.e., 
      N-formylkynurenine and kynurenine, implying Fenton chemistry in the cascade 
      (n=10). Validation of the basic data stems from successful repigmentation of skin 
      and eyelashes in affected individuals, regardless of SSV or segmental vitiligo in 
      association with NSV after reduction of epidermal H2O2 (n=5). Taken together, our 
      contribution strongly supports H2O2/ONOO-mediated stress in the pathogenesis of 
      SSV. Our findings offer new treatment intervention for lost skin and hair color.
FAU - Schallreuter, Karin U
AU  - Schallreuter KU
AD  - Institute for Pigmentary Disorders, E. M. Arndt University, Greifswald, Germany. 
      k.schallreuter@bradford.ac.uk
FAU - Salem, Mohammed A E L
AU  - Salem MA
FAU - Holtz, Sarah
AU  - Holtz S
FAU - Panske, A
AU  - Panske A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130429
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Nitrates)
RN  - 14691-52-2 (Peroxynitrous Acid)
RN  - 0 (Biopterins)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.- (pseudocatalase)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biopterins/metabolism
MH  - Catalase/*metabolism/therapeutic use
MH  - Child
MH  - Child, Preschool
MH  - Enzyme Activation/radiation effects
MH  - Epidermis/metabolism/pathology
MH  - Eyelashes/drug effects/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Hydrogen Peroxide/*metabolism
MH  - Infant
MH  - Male
MH  - Middle Aged
MH  - Nitrates/metabolism
MH  - Oxidation-Reduction/drug effects
MH  - Peroxynitrous Acid/*metabolism
MH  - Skin/drug effects/*metabolism/pathology
MH  - Skin Pigmentation/drug effects
MH  - Spectrum Analysis, Raman
MH  - Ultraviolet Rays
MH  - Vitiligo/drug therapy/*metabolism/pathology
MH  - Young Adult
OTO - NOTNLM
OT  - FT-Raman spectroscopy
OT  - MSRA
OT  - MSRB
OT  - RNS
OT  - ROS
OT  - thioredoxin reductase
EDAT- 2013/05/01 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/05/01 06:00
PHST- 2013/05/01 06:00 [entrez]
PHST- 2013/05/01 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - fj.12-226779 [pii]
AID - 10.1096/fj.12-226779 [doi]
PST - ppublish
SO  - FASEB J. 2013 Aug;27(8):3113-22. doi: 10.1096/fj.12-226779. Epub 2013 Apr 29.