PMID- 23631693
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20151119
IS  - 1939-6376 (Electronic)
IS  - 1939-6368 (Linking)
VI  - 59
IP  - 4
DP  - 2013 Aug
TI  - Anterior positioning of sex chromosomes on the head of human sperm sorted using 
      visible wavelengths.
PG  - 223-6
LID - 10.3109/19396368.2013.787129 [doi]
AB  - The human ejaculate contains subpopulations of sperm with distinct properties. 
      Human X- and Y-bearing sperm were separated with fluorescence activated cell 
      sorting. To avoid the use of UV light the quantitative DNA dyes DRAQ5(R) and 
      Dyecycle Vybrant(R) Violet were used. Sorting efficiency was similar for both 
      dyes, but lower than what is usually obtained with the classical method involving 
      Hoechst 33342 and UV light (60-70% enrichment, versus 80-90%). A total of 2,739 
      spermatozoa were evaluated, from seven distinct samples using fluorescence in 
      situ hybridization (FISH) chromosomal probes. No differences were found in sorted 
      and unsorted populations in terms of chromosome positioning, and numeric 
      chromosomal anomalies were not more evident following cell sorting. Furthermore 
      in both sorted and unsorted populations the sex chromosomes were clearly located 
      in the anterior portion of the sperm head, while a control autosome (chromosome 
      18) showed no such tendency, confirming previous findings. These results suggest 
      that other quantitative DNA dyes may be used for sex chromosome-based human sperm 
      sorting, but with lower efficiency than the standard UV-Hoechst based assay.
FAU - Alcada-Morais, Sofia
AU  - Alcada-Morais S
AD  - Biology of Reproduction and Stem Cell Group, CNC- Center for Neuroscience and 
      Cell Biology, University of Coimbra, Coimbra, Portugal
FAU - Sousa, Ana Paula
AU  - Sousa AP
FAU - Paiva, Artur
AU  - Paiva A
FAU - Almeida-Santos, Teresa
AU  - Almeida-Santos T
FAU - Ramalho-Santos, Joao
AU  - Ramalho-Santos J
LA  - eng
PT  - Journal Article
DEP - 20130501
PL  - England
TA  - Syst Biol Reprod Med
JT  - Systems biology in reproductive medicine
JID - 101464963
RN  - 0 (1,5-bis((2-(methylamino)ethyl)amino)-4,8-dihydroxyanthracene-9,10-dione)
RN  - 0 (Anthraquinones)
RN  - 0 (Coloring Agents)
SB  - IM
MH  - Anthraquinones
MH  - Cell Separation/methods
MH  - Chromosomes, Human, X/*ultrastructure
MH  - Chromosomes, Human, Y/*ultrastructure
MH  - Coloring Agents
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Sperm Head/*ultrastructure
MH  - Spermatozoa/*cytology
EDAT- 2013/05/02 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/05/02 06:00
PHST- 2013/05/02 06:00 [entrez]
PHST- 2013/05/02 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - 10.3109/19396368.2013.787129 [doi]
PST - ppublish
SO  - Syst Biol Reprod Med. 2013 Aug;59(4):223-6. doi: 10.3109/19396368.2013.787129. 
      Epub 2013 May 1.