PMID- 23632246
OWN - NLM
STAT- MEDLINE
DCOM- 20131209
LR  - 20161125
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 92
IP  - 5
DP  - 2013 Jul
TI  - Assessment of cytogenetic damage in bovine peripheral lymphocytes exposed to in 
      vitro tebuconazole-based fungicide.
PG  - 555-62
LID - S0045-6535(13)00496-7 [pii]
LID - 10.1016/j.chemosphere.2013.04.001 [doi]
AB  - The tebuconazole-based fungicide was tested in vitro for its potential genotoxic 
      and cytotoxic effects on cultured bovine peripheral lymphocytes. Following 24h 
      and 48 h of incubation, several cytogenetic endpoints were investigated such as: 
      Chromosome Aberrations (CAs); Sister Chromatid Exchanges (SCEs); Micronuclei 
      (MN); Mitotic Index (MI); Proliferation Index (PI); and Cytokinesis Block 
      Proliferation Index (CBPI). The cultured lymphocytes were exposed to the 
      fungicide formulation at concentrations of 3, 6, 15, 30 and 60 mug mL(-1). 
      Statistical significant increases were seen in the CA assays at concentrations 
      ranging from 6 to 30 mug mL(-1) for 24h. The higher doses caused a decrease or 
      total inhibition of chromosome damages in comparison to the last active dose, or 
      the control values. The Fluorescence in situ Hybridisation (FISH) technique was 
      also used for the study of stable/unstable structural chromosomal aberrations and 
      numerical aberrations of aneuploidy/polyploidy at the concentrations of 6 and 15 
      mug mL(-1). Under conditions of our study, no reciprocal translocations were 
      detected. The more frequent types of aberrations were trisomies and monosomies; 
      both have been identified in association with either bovine chromosome 5 or 7. No 
      statistical significant value was seen in the induced MN; but, the clear, evident 
      reduction of the CBPI was observed. Significant elevations of SCE were observed 
      after the applications of the fungicide formulation at doses from 15 to 60 mug 
      mL(-1) in each donor for 24h. The highest concentrations also caused a 
      statistical significant decrease in the PI. The treatment for 48 h failed to 
      exhibit any genotoxic activity of the fungicide.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Sivikova, Katarina
AU  - Sivikova K
AD  - Institute of Genetics, University of Veterinary Medicine and Pharmacy, Kosice, 
      Slovak Republic. sivikova@uvm.sk
FAU - Dianovsky, Jan
AU  - Dianovsky J
FAU - Holeckova, Beata
AU  - Holeckova B
FAU - Galdikova, Martina
AU  - Galdikova M
FAU - Kolesarova, Viera
AU  - Kolesarova V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130428
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Cytotoxins)
RN  - 0 (Fungicides, Industrial)
RN  - 0 (Mutagens)
RN  - 0 (Triazoles)
RN  - 401ATW8TRW (tebuconazole)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chromosome Aberrations/drug effects
MH  - Cytotoxins/*toxicity
MH  - Fungicides, Industrial/*toxicity
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects/pathology
MH  - Mutagens/*toxicity
MH  - Triazoles/*toxicity
EDAT- 2013/05/02 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/05/02 06:00
PHST- 2012/10/04 00:00 [received]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/04/01 00:00 [accepted]
PHST- 2013/05/02 06:00 [entrez]
PHST- 2013/05/02 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S0045-6535(13)00496-7 [pii]
AID - 10.1016/j.chemosphere.2013.04.001 [doi]
PST - ppublish
SO  - Chemosphere. 2013 Jul;92(5):555-62. doi: 10.1016/j.chemosphere.2013.04.001. Epub 
      2013 Apr 28.