PMID- 23632277
OWN - NLM
STAT- MEDLINE
DCOM- 20140218
LR  - 20231115
IS  - 1095-9890 (Electronic)
IS  - 0147-619X (Linking)
VI  - 70
IP  - 2
DP  - 2013 Sep
TI  - Characterisation of the stbD/E toxin-antitoxin system of pEP36, a plasmid of the 
      plant pathogen Erwinia pyrifoliae.
PG  - 216-25
LID - S0147-619X(13)00052-8 [pii]
LID - 10.1016/j.plasmid.2013.04.002 [doi]
AB  - pEP36 is a plasmid ubiquitously present in Erwinia pyrifoliae, a pathogen which 
      causes black stem blight of Asian pear. pEP36 is highly stable in its host, even 
      in the absence of selective pressure. The plasmid is closely related to pEA29, 
      which is widespread in E. amylovora, the causative agent of fire blight of apple 
      and pear trees. Here we report that pEP36 possesses a functional hybrid 
      toxin-antitoxin module, stbD/E(pEP36), with the toxin showing homology to the 
      RelE/ParE proteins and the antidote belonging to the Phd/YefM antitoxin family. 
      Bacteria expressing the StbE(pEP36) toxin arrest cell growth and enter a viable 
      but non-culturable stage. However, they maintain their typical cell length and do 
      not show filamentation. Pulse-chase experiments revealed that StbE(pEP36) acts as 
      a global inhibitor of protein synthesis while it does not interfere with DNA and 
      RNA synthesis. The StbD(pEP36) antitoxin is capable of neutralising StbE(pEP36) 
      toxicity. Additional experiments show that the stbD/E(pEP36) module can stabilise 
      plasmids at least 20-fold. Thus the toxin-antitoxin system may contribute to the 
      remarkable stability of pEP36.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Unterholzner, Simon J
AU  - Unterholzner SJ
AD  - Biotechnology of Horticultural Crops, Technische Universitat Munchen, 
      Liesel-Beckmann-Strasse 1, 85354 Freising, Germany. 
      unterholzner.simonjosef@gmail.com
FAU - Hailer, Barbara
AU  - Hailer B
FAU - Poppenberger, Brigitte
AU  - Poppenberger B
FAU - Rozhon, Wilfried
AU  - Rozhon W
LA  - eng
GR  - P 22734/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130428
PL  - United States
TA  - Plasmid
JT  - Plasmid
JID - 7802221
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
SB  - IM
MH  - Bacterial Proteins/*genetics
MH  - Bacterial Toxins/*genetics/toxicity
MH  - Cloning, Molecular
MH  - Erwinia/*genetics
MH  - Microscopy, Electron
MH  - Plasmids/*genetics
MH  - Protein Biosynthesis/drug effects
MH  - Pyrus/*microbiology
MH  - Species Specificity
OTO - NOTNLM
OT  - Erwinia pyrifoliae
OT  - Protein synthesis
OT  - Toxin-antitoxin
OT  - pEP36
EDAT- 2013/05/02 06:00
MHDA- 2014/02/19 06:00
CRDT- 2013/05/02 06:00
PHST- 2012/12/10 00:00 [received]
PHST- 2013/04/15 00:00 [revised]
PHST- 2013/04/17 00:00 [accepted]
PHST- 2013/05/02 06:00 [entrez]
PHST- 2013/05/02 06:00 [pubmed]
PHST- 2014/02/19 06:00 [medline]
AID - S0147-619X(13)00052-8 [pii]
AID - 10.1016/j.plasmid.2013.04.002 [doi]
PST - ppublish
SO  - Plasmid. 2013 Sep;70(2):216-25. doi: 10.1016/j.plasmid.2013.04.002. Epub 2013 Apr 
      28.