PMID- 23633297
OWN - NLM
STAT- MEDLINE
DCOM- 20140103
LR  - 20181202
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 7
DP  - 2013 Jul
TI  - Conditioned medium from human amniotic epithelial cells may induce the 
      differentiation of human umbilical cord blood mesenchymal stem cells into 
      dopaminergic neuron-like cells.
PG  - 978-86
LID - 10.1002/jnr.23225 [doi]
AB  - Dopaminergic (DA) neuron therapy has been established as a new clinical tool for 
      treating Parkinson's disease (PD). Prior to cell transplantation, there are two 
      primary issues that must be resolved: one is the appropriate seed cell origin, 
      and the other is the efficient inducing technique. In the present study, human 
      umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) were used as the 
      available seed cells, and conditioned medium from human amniotic epithelial cells 
      (ACM) was used as the inducing reagent. Results showed that the proportion of DA 
      neuron-like cells from hUCB-MSCs was significantly increased after cultured in 
      ACM, suggested by the upregulation of DAT, TH, Nurr1, and Pitx3. To identify the 
      process by which ACM induces DA neuron differentiation, we pretreated hUCB-MSCs 
      with k252a, the Trk receptor inhibitor of brain-derived neurotrophic factor 
      (BDNF) and nerve growth factor (NGF), and found that the proportion of DA 
      neuron-like cells was significantly decreased compared with ACM-treated 
      hUCB-MSCs, suggesting that NGF and BDNF in ACM were involved in the 
      differentiation process. However, we could not rule out the involvement of other 
      unidentified factors in the ACM, because ACM + k252a treatment does not fully 
      block DA neuron-like cell differentiation compared with control. The 
      transplantation of ACM-induced hUCB-MSCs could ameliorate behavioral deficits in 
      PD rats, which may be associated with the survival of engrafted DA neuron-like 
      cells. In conclusion, we propose that hUCB-MSCs are a good source of DA 
      neuron-like cells and that ACM is a potential inducer to obtain DA neuron-like 
      cells from hUCB-MSCs in vitro for an ethical and legal cell therapy for PD.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Yang, Shu
AU  - Yang S
AD  - Department of Histology and Embryology, School of Basic Medical Sciences, Capital 
      Medical University, Beijing, China.
FAU - Sun, Hai-Mei
AU  - Sun HM
FAU - Yan, Ji-Hong
AU  - Yan JH
FAU - Xue, Hong
AU  - Xue H
FAU - Wu, Bo
AU  - Wu B
FAU - Dong, Fang
AU  - Dong F
FAU - Li, Wen-Shuai
AU  - Li WS
FAU - Ji, Feng-Qing
AU  - Ji FQ
FAU - Zhou, De-Shan
AU  - Zhou DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130430
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NR4A2 protein, human)
RN  - 0 (Nuclear Receptor Subfamily 4, Group A, Member 2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 0 (homeobox protein PITX3)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - N21FAR7B4S (Apomorphine)
SB  - IM
MH  - Amnion/*cytology
MH  - Analysis of Variance
MH  - Animals
MH  - Apomorphine
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cell Differentiation/*drug effects
MH  - Culture Media, Conditioned/*pharmacology
MH  - Disease Models, Animal
MH  - Dopamine Plasma Membrane Transport Proteins/genetics/metabolism
MH  - Dopaminergic Neurons/*drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/*chemistry
MH  - Fetal Blood/*cytology
MH  - Fetus
MH  - Flow Cytometry
MH  - Homeodomain Proteins/genetics/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation/methods
MH  - Mesenchymal Stem Cells/*drug effects
MH  - Nuclear Receptor Subfamily 4, Group A, Member 2/genetics/metabolism
MH  - Oxidopamine/toxicity
MH  - Parkinson Disease/etiology/physiopathology/surgery
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkA/genetics/metabolism
MH  - Receptor, trkB/metabolism
MH  - Stereotyped Behavior/drug effects/physiology
MH  - Transcription Factors/genetics/metabolism
MH  - Tyrosine 3-Monooxygenase/genetics/metabolism
EDAT- 2013/05/02 06:00
MHDA- 2014/01/05 06:00
CRDT- 2013/05/02 06:00
PHST- 2013/01/10 00:00 [received]
PHST- 2013/02/05 00:00 [revised]
PHST- 2013/02/25 00:00 [accepted]
PHST- 2013/05/02 06:00 [entrez]
PHST- 2013/05/02 06:00 [pubmed]
PHST- 2014/01/05 06:00 [medline]
AID - 10.1002/jnr.23225 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Jul;91(7):978-86. doi: 10.1002/jnr.23225. Epub 2013 Apr 30.