PMID- 23635912
OWN - NLM
STAT- MEDLINE
DCOM- 20131217
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 31
IP  - 4-5
DP  - 2013
TI  - Human bone marrow stromal cells can differentiate to a retinal pigment epithelial 
      phenotype when co-cultured with pig retinal pigment epithelium using a transwell 
      system.
PG  - 601-13
LID - 10.1159/000350080 [doi]
AB  - BACKGROUND: There is an increasing interest in generating retinal pigment 
      epithelial (RPE) cells from stem cells for therapy against degenerative eye 
      diseases. Human bone marrow stromal cells (hBMSCs) can be induced to express 
      retinal neuron-specific markers when co-cultured with retinal neurons, however, 
      whether hBMSCs can differentiate into RPE-like cells in a co-culture system has 
      not been clarified. METHODS: The induction of hBMSCs into RPE-like cells was 
      performed by combining hBMSCs and pig RPE cells in a transwell system. The 
      biomarkers of hBMSCs-derived RPE cells were determined by quantitative RT-PCR and 
      immunofluorescence. The function of induced cells was assayed by ELISA for 
      secretion of neurotrophic factors. RESULTS: Intracellular pigment granules and 
      many RPE markers existed in hBMSCs-derived RPE cells after co-culturing with pig 
      RPE cells for 14 days. Typical RPE functions, such as phagocytosis of 
      photoreceptor outer segments and secretion of the trophic factors, brain-derived 
      neurotrophic factor (BDNF) and glia-derived neurotrophic factor (GDNF), were 
      observed in these induced cells. CONCLUSION: hBMSCs can be induced toward 
      functional RPE cells simply by transwell-based co-culture with RPE cells.
CI  - Copyright (c) 2013 S. Karger AG, Basel.
FAU - Duan, Ping
AU  - Duan P
AD  - Southwest Eye Hospital, Southwest Hospital, Third Military Medical University, 
      Chongqing, China.
FAU - Xu, Haiwei
AU  - Xu H
FAU - Zeng, Yuxiao
AU  - Zeng Y
FAU - Wang, Yi
AU  - Wang Y
FAU - Yin, Zheng Qin
AU  - Yin ZQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130502
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/*cytology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques/instrumentation
MH  - Glial Cell Line-Derived Neurotrophic Factor/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Nerve Growth Factors/metabolism
MH  - Phagocytosis
MH  - Phenotype
MH  - Retinal Pigment Epithelium/*cytology/metabolism
MH  - Swine
EDAT- 2013/05/03 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/05/03 06:00
PHST- 2013/04/16 00:00 [accepted]
PHST- 2013/05/03 06:00 [entrez]
PHST- 2013/05/03 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - 000350080 [pii]
AID - 10.1159/000350080 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2013;31(4-5):601-13. doi: 10.1159/000350080. Epub 2013 May 
      2.