PMID- 23636823
OWN - NLM
STAT- MEDLINE
DCOM- 20130924
LR  - 20211021
IS  - 1465-2099 (Electronic)
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 94
IP  - Pt 8
DP  - 2013 Aug
TI  - Multiple effects of dendritic cell depletion on murine norovirus infection.
PG  - 1761-1768
LID - 10.1099/vir.0.052134-0 [doi]
AB  - Dendritic cells (DCs) are permissive to murine norovirus (MNV) infection in vitro 
      and in vivo. However, their roles during infection in vivo are not well defined. 
      To determine the role of DCs during infection, conventional DCs were depleted 
      from CD11c-DTR mice and infected with a persistent MNV strain. Viral titres in 
      the intestine and secondary lymphoid organs were determined at early time points 
      during infection, and anti-MNV antibody responses were analysed later during 
      infection. Depletion of conventional DCs resulted in increased viral loads in 
      intestinal tissues, impaired generation of antibody responses, and a failure of 
      MNV to efficiently infect lymphoid tissues. These data suggest that DCs play 
      multiple roles in MNV pathogenesis, in both innate immunity and the efficient 
      generation of adaptive immune responses against MNV, as well as by promoting the 
      dissemination of MNV to secondary lymphoid tissues. This is the first study to 
      probe the roles of DCs in controlling and/or facilitating a norovirus infection 
      in vivo and provides the basis for further studies aimed at defining mechanisms 
      by which DCs control MNV replication and promote viral dissemination.
FAU - Elftman, Michael D
AU  - Elftman MD
AD  - Department of Biomedical and Diagnostic Sciences, University of Detroit Mercy 
      School of Dentistry, Detroit, Michigan 48208, USA.
AD  - Department of Microbiology and Immunology, University of Michigan Medical School, 
      Ann Arbor, Michigan 48109, USA.
FAU - Gonzalez-Hernandez, Mariam B
AU  - Gonzalez-Hernandez MB
AD  - Department of Microbiology and Immunology, University of Michigan Medical School, 
      Ann Arbor, Michigan 48109, USA.
FAU - Kamada, Nobuhiko
AU  - Kamada N
AD  - Department of Pathology and Comprehensive Cancer Center, University of Michigan 
      Medical School, Ann Arbor, Michigan 48109, USA.
FAU - Perkins, Cheryl
AU  - Perkins C
AD  - Molecular Diagnostics Research, Animal Diagnostic Services, Charles River 
      Laboratories, Wilmington, Massachusetts 01887, USA.
FAU - Henderson, Kenneth S
AU  - Henderson KS
AD  - Molecular Diagnostics Research, Animal Diagnostic Services, Charles River 
      Laboratories, Wilmington, Massachusetts 01887, USA.
FAU - Nunez, Gabriel
AU  - Nunez G
AD  - Department of Pathology and Comprehensive Cancer Center, University of Michigan 
      Medical School, Ann Arbor, Michigan 48109, USA.
FAU - Wobus, Christiane E
AU  - Wobus CE
AD  - Department of Microbiology and Immunology, University of Michigan Medical School, 
      Ann Arbor, Michigan 48109, USA.
LA  - eng
GR  - R01 AI080611/AI/NIAID NIH HHS/United States
GR  - R01 DK091191/DK/NIDDK NIH HHS/United States
GR  - DK091191/DK/NIDDK NIH HHS/United States
GR  - AI080611/AI/NIAID NIH HHS/United States
GR  - 2 T32AI007413-18/AI/NIAID NIH HHS/United States
GR  - T32 AI007413/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130501
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Caliciviridae Infections/*immunology
MH  - Dendritic Cells/*immunology
MH  - Disease Models, Animal
MH  - Intestines/virology
MH  - Leukocyte Reduction Procedures
MH  - Lymphoid Tissue/virology
MH  - Mice
MH  - Norovirus/*immunology
MH  - Viral Load
PMC - PMC3749526
EDAT- 2013/05/03 06:00
MHDA- 2013/09/26 06:00
PMCR- 2014/08/01
CRDT- 2013/05/03 06:00
PHST- 2013/05/03 06:00 [entrez]
PHST- 2013/05/03 06:00 [pubmed]
PHST- 2013/09/26 06:00 [medline]
PHST- 2014/08/01 00:00 [pmc-release]
AID - 052134 [pii]
AID - 10.1099/vir.0.052134-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2013 Aug;94(Pt 8):1761-1768. doi: 10.1099/vir.0.052134-0. Epub 2013 
      May 1.