PMID- 23643543
OWN - NLM
STAT- MEDLINE
DCOM- 20140107
LR  - 20130528
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 148
IP  - 1
DP  - 2013 Jun 21
TI  - Pharmacokinetic study of four flavones of Glycyrrhiza in rat plasma using 
      HPLC-MS.
PG  - 266-70
LID - S0378-8741(13)00277-8 [pii]
LID - 10.1016/j.jep.2013.04.024 [doi]
AB  - AIM OF THE STUDY: This study aimed to develop a specific HPLC-MS method for 
      simultaneous quantification of four flavones of Glycyrrhiza in rat plasma after 
      oral administration and to describe the pharmacokinetics of four flavones in rat 
      plasma. MATERIALS AND METHODS: A simple, sensitive and selective method for 
      simultaneous determination of four flavones of Glycyrrhiza in rat plasma, i.e., 
      liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin, by high 
      performance liquid chromatography-tandem mass spectrometry (HPLC-MS) with 
      negative electrospray ionization mode, was developed and validated. The method 
      was applied to investigate the pharmacokinetics of four flavones in rat plasma 
      after oral administration of Glycyrrhiza flavones. Chromatographic separation was 
      accomplished on an Agilent TC-C18 column (4.6mmx250mm, and 5mum), with gradient 
      elution by using a mixture of methanoic acid (A) and acetonitrile (B) as the 
      mobile phase at a flow rate of 0.8mL/min. RESULTS: The calibration curves for 
      four flavones had good linearity higher than 0.997 in the measured range. 
      Relative standard deviations (RSDs) of the intra- and inter-day precision at 
      different levels were all less than 4.8%. The pharmacokinetic profile of four 
      flavones in rat plasma was fitted with a two-compartment model detected by a 
      simple, rapid and accurate HPLC-MS method. Time (h) to reach peak concentration 
      (mug/mL) of liquiritin (2.69+/-0.04), isoliquiritin (10.16+/-0.02), liquiritigenin 
      (2.83+/-0.02), and isoliquiritigenin (0.28+/-0.01) was 2.02+/-0.23, 1.97+/-0.20, 
      0.48+/-0.02, and 1.93+/-0.36, respectively. The distribution and elimination 
      half-life (h) and area under the concentration-time curve (mug/mL-h) from t=0 to 
      last time of liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin 
      were 1.02+/-0.48/2.27+/-0.53/16.97+/-0.43, 2.04+/-1.01/2.38+/-0.80/69.20+/-5.24, 
      0.35+/-0.10/4.26+/-0.16/14.83+/-0.11, and 1.18+/-0.32/3.04+/-0.22/2.10+/-0.09, respectively. 
      Isoliquiritin presented the phenomenon of double peaks and the others appeared 
      together in a single and plateau absorption phase. Isoliquiritigenin had the 
      lowest oral bioavailability because of Cmax and AUC0-infinity. Liquiritigenin had the 
      fastest absorption and distribution rate and the lowest elimination rate 
      according to Tmax, t1/2alpha, and t1/2beta. CONCLUSIONS: This paper first reported on 
      identification and determination of four flavones of Glycyrrhiza in rat plasma 
      and their respective pharmacokinetic characteristics. The results provided a 
      meaningful basis for better understanding the absorption mechanism of Glycyrrhiza 
      and evaluating the clinical application of this medicine.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Wu, Yin-Ping
AU  - Wu YP
AD  - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 
      116600, China.
FAU - Meng, Xian-Sheng
AU  - Meng XS
FAU - Bao, Yong-Rui
AU  - Bao YR
FAU - Wang, Shuai
AU  - Wang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130430
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Flavones)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Flavones/blood/*pharmacokinetics
MH  - *Glycyrrhiza
MH  - Mass Spectrometry
MH  - Plant Roots
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2013/05/07 06:00
MHDA- 2014/01/08 06:00
CRDT- 2013/05/07 06:00
PHST- 2012/10/23 00:00 [received]
PHST- 2013/04/12 00:00 [revised]
PHST- 2013/04/13 00:00 [accepted]
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2014/01/08 06:00 [medline]
AID - S0378-8741(13)00277-8 [pii]
AID - 10.1016/j.jep.2013.04.024 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Jun 21;148(1):266-70. doi: 10.1016/j.jep.2013.04.024. Epub 
      2013 Apr 30.