PMID- 2364362
OWN - NLM
STAT- MEDLINE
DCOM- 19900813
LR  - 20190619
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 66
IP  - 3
DP  - 1990 Aug 1
TI  - Targeted cytogenetic analysis of gastric tumors by in situ hybridization with a 
      set of chromosome-specific DNA probes.
PG  - 491-7
AB  - Fluorescent in situ hybridization (FISH) with biotinated chromosome-specific 
      repetitive DNA probes was used for the cytogenetic study of ten gastric 
      adenocarcinomas. All tumors (eight male, two female patients) were histologically 
      moderately or poorly differentiated and nine of ten had metastasized to regional 
      lymph nodes. The authors applied a set of satellite DNA probes, specific for 
      chromosomes 1, 7, 17, X, and Y in order to detect numerical chromosome 
      aberrations in freshly isolated tumor cell nuclei. Normal diploid human 
      lymphocyte nuclei and, in a number of cases, normal gastric mucosa served as 
      controls. Parallel with the hybridization experiments DNA flow cytometric study 
      of acridine orange (AO)-stained tumor cells was carried out. By means of FISH the 
      authors found seven cases to be aneuploid, the other three cases appeared 
      diploid. This was confirmed by DNA flow cytometric analysis with AO. Furthermore, 
      loss of the Y chromosome in a high percentage of cells was seen by FISH in six of 
      eight tumors from male patients. In the other two male samples a possible loss 
      was observed in a small proportion of cells (15%). In three patients from whom 
      the authors had normal gastric mucosa the Y loss was restricted to the tumor 
      cells. These data indicate that in situ hybridization with chromosome-specific 
      repetitive DNA probes can serve as a cytogenetic tool for the analysis of 
      interphase nuclei of solid human tumors, at least with respect to the detection 
      of numerical chromosome abnormalities.
FAU - van Dekken, H
AU  - van Dekken H
AD  - Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, New 
      York.
FAU - Pizzolo, J G
AU  - Pizzolo JG
FAU - Kelsen, D P
AU  - Kelsen DP
FAU - Melamed, M R
AU  - Melamed MR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Aged
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - *DNA Probes
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Nucleic Acid Hybridization
MH  - Ploidies
MH  - Repetitive Sequences, Nucleic Acid
MH  - Stomach Neoplasms/*genetics
MH  - X Chromosome
MH  - Y Chromosome
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1002/1097-0142(19900801)66:3<491::aid-cncr2820660315>3.0.co;2-q [doi]
PST - ppublish
SO  - Cancer. 1990 Aug 1;66(3):491-7. doi: 
      10.1002/1097-0142(19900801)66:3<491::aid-cncr2820660315>3.0.co;2-q.