PMID- 23643896
OWN - NLM
STAT- MEDLINE
DCOM- 20140108
LR  - 20130610
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 546
DP  - 2013 Jun 24
TI  - Stimulatory effect of gonadal hormones on fetal rat hippocampal neural 
      proliferation requires neurotrophin receptor activation in vitro.
PG  - 1-5
LID - S0304-3940(13)00378-9 [pii]
LID - 10.1016/j.neulet.2013.04.029 [doi]
AB  - To determine the effects of gonadal hormones on proliferation of the hippocampal 
      neural cells, which are of importance in learning and memory function. 
      17beta-Estradiol or testosterone was added to the culture at various concentrations. 
      Their proliferation and protective effects on the neural cell were determined 
      with BrdU, flow cytometry and MTT assay. Effects of the gonadal hormones on 
      brain-derived neurotrophic factor (BDNF) expression were determined using ELISA 
      and RT-PCR respectively. 17beta-Estradiol and testosterone at 20nM or higher 
      concentrations significantly increased the neural cell proliferation and 
      viability, and induced increasing in the S phase arrest which is essential for 
      cell proliferation. Both estradiol and testosterone significantly increased the 
      neural cell expression of cellular mature BDNF and BDNF mRNA. Effect of 
      testosterone on hippocampal neural proliferation was blocked by Trk neurotrophin 
      receptor inhibitor. 17beta-Estradiol and testosterone promoted hippocampal neural 
      proliferation and improved cell viability in vitro. The effect of testosterone on 
      hippocampal neural cell proliferation required neurotrophin receptor activation.
CI  - Copyright (c) 2013 The Authors. Published by Elsevier Ireland Ltd.. All rights 
      reserved.
FAU - Pan, Meixia
AU  - Pan M
AD  - Department of Nutrition, Guangdong General Hospital, Guangdong Academy of Medical 
      Sciences, Guangzhou 510080, China. meixpan@yahoo.com.cn
FAU - Zhang, Caixia
AU  - Zhang C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130501
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Gonadal Hormones)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Estradiol/*administration & dosage
MH  - Gonadal Hormones/administration & dosage
MH  - Hippocampus/drug effects/*embryology/*physiology
MH  - Neurons/drug effects/*physiology
MH  - Rats
MH  - Receptors, Nerve Growth Factor/*metabolism
MH  - Testosterone/*administration & dosage
EDAT- 2013/05/07 06:00
MHDA- 2014/01/09 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/02/11 00:00 [received]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/04/12 00:00 [accepted]
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2014/01/09 06:00 [medline]
AID - S0304-3940(13)00378-9 [pii]
AID - 10.1016/j.neulet.2013.04.029 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 Jun 24;546:1-5. doi: 10.1016/j.neulet.2013.04.029. Epub 2013 
      May 1.