PMID- 23643932
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20131121
IS  - 1873-2968 (Electronic)
IS  - 0006-2952 (Linking)
VI  - 85
IP  - 12
DP  - 2013 Jun 15
TI  - Heterologously expressed formyl peptide receptor 2 (FPR2/ALX) does not respond to 
      lipoxin A(4).
PG  - 1795-802
LID - S0006-2952(13)00261-X [pii]
LID - 10.1016/j.bcp.2013.04.019 [doi]
AB  - Lipoxin A(4) (LXA(4)) has been described as an anti-inflammatory mediator, which 
      exerts its effects through the formyl peptide receptor FPR2, also known as ALX. 
      However, there has been a controversy whether or not cells expressing FPR2/ALX, 
      such as neutrophils, respond to LXA(4). We, therefore, systematically examined the 
      ability of the human and murine forms of the receptor to respond to LXA(4). We show 
      that both receptor orthologues responded to the FPR2/ALX peptide agonist WKYMVM 
      when expressed heterologously. In contrast, LXA(4) from different sources neither 
      increased [Ca(2)(+)](i) and extracellular-signal-regulated kinase (ERK) 
      phosphorylation, nor did it induce a decrease in cAMP levels or a translocation 
      of beta-arrestin. Also, several LXA(4) analogs were found to be unable to signal 
      through FPR2/ALX. We conclude that FPR2/ALX is not activated by LXA(4) and that the 
      molecular mechanism by which LXA(4) functions still needs to be identified.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Hanson, Julien
AU  - Hanson J
AD  - Department of Pharmacology, Max Planck Institute for Heart and Lung Research, 
      Ludwigstrasse 43, 61231 Bad Nauheim, Germany. j.hanson@ulg.ac.be
FAU - Ferreiros, Nerea
AU  - Ferreiros N
FAU - Pirotte, Bernard
AU  - Pirotte B
FAU - Geisslinger, Gerd
AU  - Geisslinger G
FAU - Offermanns, Stefan
AU  - Offermanns S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130501
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (FPR2 protein, human)
RN  - 0 (Fpr1 protein, mouse)
RN  - 0 (HSH2 protein, mouse)
RN  - 0 (Lipoxins)
RN  - 0 (Receptors, Formyl Peptide)
RN  - 0 (Receptors, Lipoxin)
RN  - 0 (lipoxin A4)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - Cyclic AMP/antagonists & inhibitors/metabolism
MH  - *Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Lipoxins/metabolism/*physiology
MH  - Mice
MH  - Receptors, Formyl Peptide/*administration & 
      dosage/biosynthesis/*metabolism/physiology
MH  - Receptors, Lipoxin/biosynthesis/*metabolism
EDAT- 2013/05/07 06:00
MHDA- 2013/08/10 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/02/13 00:00 [received]
PHST- 2013/04/18 00:00 [revised]
PHST- 2013/04/23 00:00 [accepted]
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - S0006-2952(13)00261-X [pii]
AID - 10.1016/j.bcp.2013.04.019 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 2013 Jun 15;85(12):1795-802. doi: 10.1016/j.bcp.2013.04.019. 
      Epub 2013 May 1.