PMID- 23644108
OWN - NLM
STAT- MEDLINE
DCOM- 20140717
LR  - 20130506
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 33
IP  - 4
DP  - 2013 Apr
TI  - [Effect of homocysteine on gluconeogenesis in mice].
PG  - 507-10
AB  - OBJECTIVE: To investigate the expressions of glucose-6-phosphatase (G6Pase) and 
      phosphoenolpyruvate carboxykinase (PEPCK) in the liver of mice with 
      hyperhomocysteinemia (HHcy) and explore the mechanism of gluconeogenesis induced 
      by homocysteine. METHODS: Fifty mice were randomly divided into normal control 
      group (n=25) and HHcy group (n=25) and fed with normal food and food supplemented 
      with 1.5% methionine, respectively. After 3 months of feeding, the fasting blood 
      glucose and insulin levels were determined, and HOMA insulin resistance index 
      (HOMA-IR) was calculated. The expressions of G6Pase and PEPCK in the liver of 
      mice were detected using RT-PCR and Western blotting. RESULTS: The fasting blood 
      glucose and insulin levels and HOMA-IR were significantly higher in HHcy group 
      than in the control group (P<0.05). RT-PCR and Western blotting showed that the 
      hepatic expressions of G6Pase and PEPCK mRNA and proteins increased significantly 
      in HHcy group compared with those in the control group (P<0.05). CONCLUSION: 
      Homocysteine promotes gluconeogenesis to enhance glucose output and contribute to 
      the occurrence of insulin resistance.
FAU - Wang, Yanan
AU  - Wang Y
AD  - Department of Pathology and Pathophysiology, Kunming Medical University, China. 
      562768905@qq.com
FAU - Yang, Lijuan
AU  - Yang L
FAU - Wang, Welin
AU  - Wang W
FAU - Feng, Weiyang
AU  - Feng W
FAU - Gui, Li
AU  - Gui L
FAU - Wang, Fang
AU  - Wang F
FAU - Li, Shude
AU  - Li S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - EC 3.1.3.9 (Glucose-6-Phosphatase)
RN  - EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP))
SB  - IM
MH  - Animals
MH  - *Gluconeogenesis
MH  - Glucose-6-Phosphatase/*metabolism
MH  - Homocysteine/*blood
MH  - Hyperhomocysteinemia/metabolism
MH  - Insulin Resistance
MH  - Liver/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Phosphoenolpyruvate Carboxykinase (ATP)/*metabolism
EDAT- 2013/05/07 06:00
MHDA- 2014/07/18 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2014/07/18 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2013 Apr;33(4):507-10.