PMID- 23644274 OWN - NLM STAT- MEDLINE DCOM- 20131108 LR - 20181202 IS - 1873-6424 (Electronic) IS - 0269-7491 (Linking) VI - 179 DP - 2013 Aug TI - Metabolic and molecular methods to evaluate the organoclay effects on a bacterial community. PG - 39-44 LID - S0269-7491(13)00213-3 [pii] LID - 10.1016/j.envpol.2013.04.012 [doi] AB - The aim of this work was to evaluate the influence exerted by two different commercial organoclays (DELLITE 43B and DELLITE 67G) on a model microbial consortium using microbial metabolic characterization with BIOLOG system and denaturing gradient gel electrophoresis (DGGE) molecular approach. The information obtained from the molecular analyses, in their complex, account for the differences in species composition induced on the reference consortium by the contact with the organoclays under study. DELLITE 43B resulted to produce a marked selective effect, stimulating the quantitative increase especially of Pseudomonas pseudoalcaligenes. A weaker effect was found for DELLITE 67G. On the other hand, Biolog analyses indicated a depressing action exerted by DELLITE 43B on the metabolic activity of the model microbial consortium as a whole. The presence of P. pseudoalcaligenes and B. borstelensis in the bacterial community after the treatments confirmed that a positive change in the microbial structure consortium occurred. CI - Copyright (c) 2013 Elsevier Ltd. All rights reserved. FAU - Abbate, Cristina AU - Abbate C AD - Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, University of Catania, Via Santa Sofia 98, 95123 Catania, Italy. cristina.abbate@unict.it FAU - Ambrosoli, Roberto AU - Ambrosoli R FAU - Minati, Jose Luis AU - Minati JL FAU - Gennari, Mara AU - Gennari M FAU - Arena, Maria AU - Arena M LA - eng PT - Journal Article DEP - 20130503 PL - England TA - Environ Pollut JT - Environmental pollution (Barking, Essex : 1987) JID - 8804476 RN - 0 (Aluminum Silicates) RN - 0 (Soil) RN - 0 (Soil Pollutants) RN - T1FAD4SS2M (Clay) SB - IM MH - Aluminum Silicates/chemistry/*metabolism MH - Bacteria/growth & development/*metabolism MH - Clay MH - Ecosystem MH - Environmental Restoration and Remediation/methods MH - Microbial Consortia/*physiology MH - Soil/chemistry MH - *Soil Microbiology MH - Soil Pollutants/chemistry/*metabolism EDAT- 2013/05/07 06:00 MHDA- 2013/11/10 06:00 CRDT- 2013/05/07 06:00 PHST- 2012/11/12 00:00 [received] PHST- 2013/03/12 00:00 [revised] PHST- 2013/04/06 00:00 [accepted] PHST- 2013/05/07 06:00 [entrez] PHST- 2013/05/07 06:00 [pubmed] PHST- 2013/11/10 06:00 [medline] AID - S0269-7491(13)00213-3 [pii] AID - 10.1016/j.envpol.2013.04.012 [doi] PST - ppublish SO - Environ Pollut. 2013 Aug;179:39-44. doi: 10.1016/j.envpol.2013.04.012. Epub 2013 May 3.