PMID- 23644770
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20130819
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 97
IP  - 17
DP  - 2013 Sep
TI  - Generation of biologically contained, readily transformable, and genetically 
      manageable mutants of the biotechnologically important Bacillus pumilus.
PG  - 7805-19
LID - 10.1007/s00253-013-4935-5 [doi]
AB  - Bacillus pumilus mutants were generated by targeted deletion of a set of genes 
      eventually facilitating genetic handling and assuring biological containment. The 
      well-defined and stable mutants do not form functional endospores due to the 
      deletion of yqfD, an essential sporulation gene; they are affected in DNA repair, 
      as DeltauvrBA rendered them UV hypersensitive and, thus, biologically contained; they 
      are deficient for the uracil phosphoribosyl-transferase (Deltaupp), allowing for 
      5-fluorouracil-based counterselection facilitating rapid allelic exchanges; and 
      they are readily transformable due to the deletion of the restrictase encoding 
      locus (DeltahsdR) of a type I restriction modification system. Vegetative growth as 
      well as extracellular enzyme production and secretion are in no case affected. 
      The combination of such gene deletions allows for development of B. pumilus 
      strains suited for industrial use and further improvements.
FAU - Wemhoff, Stephanie
AU  - Wemhoff S
AD  - Institut fur Molekulare Mikrobiologie und Biotechnologie, Westfalische 
      Wilhelms-Universitat Munster, Corrensstrasse 3, 48149, Munster, Germany.
FAU - Meinhardt, Friedhelm
AU  - Meinhardt F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130505
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
SB  - IM
MH  - Bacillus/*genetics/growth & development/metabolism
MH  - Gene Deletion
MH  - Genetic Engineering
MH  - *Industrial Microbiology
MH  - *Sequence Deletion
MH  - Spores, Bacterial/genetics/growth & development/metabolism
MH  - *Transformation, Genetic
EDAT- 2013/05/07 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/02/26 00:00 [received]
PHST- 2013/04/15 00:00 [accepted]
PHST- 2013/04/12 00:00 [revised]
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
AID - 10.1007/s00253-013-4935-5 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2013 Sep;97(17):7805-19. doi: 
      10.1007/s00253-013-4935-5. Epub 2013 May 5.