PMID- 23644893 OWN - NLM STAT- MEDLINE DCOM- 20140623 LR - 20211021 IS - 1568-5608 (Electronic) IS - 0925-4692 (Linking) VI - 21 IP - 6 DP - 2013 Dec TI - Comparison of the ability of chondroitin sulfate derived from bovine, fish and pigs to suppress human osteoclast activity in vitro. PG - 407-12 LID - 10.1007/s10787-013-0171-y [doi] AB - Chondroitin sulfate (CS) compounds are commonly used to manage OA symptoms. Recent literature has indicated that abnormal subchondral bone metabolism may have a role in the pathogenesis of OA. The aim of this study was to access the effects of chondroitin sulfate obtained from bovine, fish and porcine sources on human osteoclast formation and activity in vitro. Human osteoclasts were generated from blood mononuclear cells. Cells were cultured over 17 days with the addition of macrophage colony stimulating factor (M-CSF) and then stimulated with receptor activator of nuclear factor kappa B ligand from day 7. Cells were treated with the CS commencing from day 7 onwards. To assess effects on osteoclasts, tartrate resistant acid phosphatate (TRAP) expression and resorption of whale dentine assays were used. Bovine-derived CS consistently suppressed osteoclast activity at concentrations as low as 1 mug/ml. Fish and porcine CS was less consistent in their effects varying with different donor cells. All CS compounds had little effect on TRAP activity. mRNA analysis using real-time PCR of bovine CS treated cells indicated that the inhibition of activity was not due to inhibition of the late stage NFATc1 transcription factor (p > 0.05). These results are consistent with CS inhibition of mature osteoclast activity rather than the formation of mature osteoclasts. It would appear that there are differences in activity of the different CS compounds with bovine-derived CS being the most consistently effective inhibitor of osteoclast resorption, but the results need to be confirmed. FAU - Cantley, M D AU - Cantley MD AD - Discipline of Anatomy and Pathology, School of Medical Sciences, University of Adelaide, Adelaide, SA, Australia. FAU - Rainsford, K D AU - Rainsford KD FAU - Haynes, D R AU - Haynes DR LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130504 PL - Switzerland TA - Inflammopharmacology JT - Inflammopharmacology JID - 9112626 RN - 0 (Bone Density Conservation Agents) RN - 0 (Isoenzymes) RN - 0 (RANK Ligand) RN - 0 (Recombinant Proteins) RN - 0 (TNFSF11 protein, human) RN - 81627-83-0 (Macrophage Colony-Stimulating Factor) RN - 9007-28-7 (Chondroitin Sulfates) RN - EC 3.1.3.2 (Acid Phosphatase) RN - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase) SB - IM MH - Acid Phosphatase/metabolism MH - Animals MH - Bone Density Conservation Agents/adverse effects/*metabolism MH - Cattle MH - Cell Survival MH - Cell Transdifferentiation MH - Cells, Cultured MH - Chondroitin Sulfates/adverse effects/*metabolism MH - Dentin/metabolism/ultrastructure MH - *Dietary Supplements/adverse effects MH - *Down-Regulation MH - Fishes MH - Humans MH - In Vitro Techniques MH - Isoenzymes/metabolism MH - Leukocytes, Mononuclear/cytology/enzymology/metabolism MH - Macrophage Colony-Stimulating Factor/genetics/metabolism MH - Osteoclasts/cytology/enzymology/*metabolism MH - RANK Ligand/genetics/metabolism MH - Recombinant Proteins/metabolism MH - Reproducibility of Results MH - Sus scrofa MH - Tartrate-Resistant Acid Phosphatase MH - Tooth Resorption/metabolism/pathology/prevention & control MH - Whales EDAT- 2013/05/07 06:00 MHDA- 2014/06/24 06:00 CRDT- 2013/05/07 06:00 PHST- 2013/04/04 00:00 [received] PHST- 2013/04/15 00:00 [accepted] PHST- 2013/05/07 06:00 [entrez] PHST- 2013/05/07 06:00 [pubmed] PHST- 2014/06/24 06:00 [medline] AID - 10.1007/s10787-013-0171-y [doi] PST - ppublish SO - Inflammopharmacology. 2013 Dec;21(6):407-12. doi: 10.1007/s10787-013-0171-y. Epub 2013 May 4.