PMID- 23645222
OWN - NLM
STAT- MEDLINE
DCOM- 20140206
LR  - 20211021
IS  - 1573-6903 (Electronic)
IS  - 0364-3190 (Linking)
VI  - 38
IP  - 8
DP  - 2013 Aug
TI  - Rotenone could activate microglia through NFkappaB associated pathway.
PG  - 1553-60
LID - 10.1007/s11064-013-1055-7 [doi]
AB  - Parkinson's disease (PD) is a common neurodegenerative disease, and its etiology 
      remains obscure. Increasing evidence has suggested an important role for 
      environmental factors such as exposure to pesticides in increasing the risk of 
      developing PD and inflammation is the early incident during the process of PD. In 
      this study, we measure the pro-inflammatory cytokines by enzyme-linked 
      immunosorbnent assay and RT-PCR methods; analyze the reactive oxygen species by 
      DCFH-DA; detected nuclear factor kappaB (NFkappaB) translocation by western blot and 
      immunofluorescence methods; and analyze the phosphorylation of mitogen-activated 
      protein (MAP) kinase and protein level of Nurr1 by western blot. Results showed 
      that rotenone could induce tumor neurosis factor alpha (TNFalpha) and interleukin 1beta 
      (IL-1beta) release from BV-2 cells, enhance TNFalpha and IL-1beta mRNA levels in substantia 
      nigra lesioned by rotenone; also, rotenone could increase the phosphorylation of 
      inhibitor of kappaB (IkappaB), extracellular regulated protein kinase , c-Jun N-terminal 
      kinase, p38 MAP kinases and promote p65 subunit of NFkappaB translocation to nuclear; 
      at the same time, rotenone could decrease the protein level of Nurr1 in nuclear. 
      So, rotenone exerted toxicity through activating microglia, and its mechanism 
      might be associated with NFkappaB signal pathway.
FAU - Yuan, Yu-he
AU  - Yuan YH
AD  - State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, 
      Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation 
      Study, Department of Pharmacology, Institute of Materia Medica, Chinese Academy 
      of Medical Sciences and Peking Union Medical College, Xiannongtan Street, Xuanwu 
      District, Beijing, 100050, People's Republic of China.
FAU - Sun, Jian-dong
AU  - Sun JD
FAU - Wu, Miao-miao
AU  - Wu MM
FAU - Hu, Jin-feng
AU  - Hu JF
FAU - Peng, Shan-ying
AU  - Peng SY
FAU - Chen, Nai-Hong
AU  - Chen NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130507
PL  - United States
TA  - Neurochem Res
JT  - Neurochemical research
JID - 7613461
RN  - 0 (DNA Primers)
RN  - 0 (NF-kappa B)
RN  - 03L9OT429T (Rotenone)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - DNA Primers
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microglia/*drug effects/metabolism
MH  - NF-kappa B/*metabolism
MH  - Polymerase Chain Reaction
MH  - Rotenone/*pharmacology
EDAT- 2013/05/07 06:00
MHDA- 2014/02/07 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/02/22 00:00 [received]
PHST- 2013/04/18 00:00 [accepted]
PHST- 2013/03/26 00:00 [revised]
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2014/02/07 06:00 [medline]
AID - 10.1007/s11064-013-1055-7 [doi]
PST - ppublish
SO  - Neurochem Res. 2013 Aug;38(8):1553-60. doi: 10.1007/s11064-013-1055-7. Epub 2013 
      May 7.