PMID- 23645739
OWN - NLM
STAT- MEDLINE
DCOM- 20130626
LR  - 20141120
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 33
IP  - 5
DP  - 2013 May
TI  - Expression of bone morphogenetic protein-10 (BMP10) in human urothelial cancer of 
      the bladder and its effects on the aggressiveness of bladder cancer cells in 
      vitro.
PG  - 1917-25
AB  - BACKGROUND: Bone morphogenetic protein-10 (BMP10), a novel member of the bone 
      morphogenetic protein (BMP) family, has been indicated as a possible tumour 
      suppressor in prostate and breast cancer. However, its role in urothelial tumours 
      remains unknown. In the present study, we examined the role of BMP10 in 
      urothelial cancer cells and the expression of BMP10 in human urethelial cancer of 
      the bladder. MATERIALS AND METHODS: The expression of BMP10 was examined in human 
      bladder tissues and in the T24 human bladder cancer cell line using 
      immunochemical staining and reverse transcription polymerase chain reaction 
      (RT-PCR), respectively. The biological impact of modifying BMP10 expression, 
      through genetic manipulation, in urothelial cancer cells was evaluated using in 
      vitro models. RESULTS: mRNA for BMP10 and receptors of BMPs was expressed in T24 
      cell lines. BMP10 protein expression was observed in normal urothelial and 
      stromal cells, but was found to be decreased in or absent from urothelial cancer 
      cells. The frequency of positive staining in normal tissues (9/9) was 
      significantly higher than that in urothelial cancer tissues (6/15) (p=0.007). T24 
      cells were transfected with BMP10 expression plasmid. It was further demonstrated 
      that overexpression of BMP10 reduced the growth rate of T24 cells, and markedly 
      reduced the motility, and adhesion of T24 cells in vitro. No significant effects 
      were seen on in vitro invasiveness of T24 cells following BMP10 transfection. 
      CONCLUSION: Expression of BMP10 protein is reduced in cancer cells of bladder 
      tumours. Overexpression of BMP10 has an inhibitory effect on the growth, 
      adhesion, and migration of bladder cancer cells in vitro. This would suggest a 
      potential tumour suppressor role of BMP10 in bladder cancer.
FAU - Zhang, Ning
AU  - Zhang N
AD  - Department of Urology, Beijing Chaoyang Hospital, Capital Medical University No. 
      8, Gongti South Road, Chaoyang District, Beijing 100020, P.R. China. 
      niru7429@yahoo.com.cn
FAU - Ye, Lin
AU  - Ye L
FAU - Wu, Liyang
AU  - Wu L
FAU - Deng, Xiaohu
AU  - Deng X
FAU - Yang, Yong
AU  - Yang Y
FAU - Jiang, Wen G
AU  - Jiang WG
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (BMP10 protein, human)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Apoptosis
MH  - Blotting, Western
MH  - Bone Morphogenetic Proteins/genetics/*metabolism
MH  - Cell Adhesion
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - In Vitro Techniques
MH  - RNA, Messenger/genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Cells, Cultured
MH  - Urinary Bladder/metabolism/*pathology
MH  - Urinary Bladder Neoplasms/genetics/metabolism/*pathology
OTO - NOTNLM
OT  - BMP10
OT  - T24 cells
OT  - growth
OT  - motility and adhesion
OT  - urothelial cancer of bladder
EDAT- 2013/05/07 06:00
MHDA- 2013/06/28 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2013/06/28 06:00 [medline]
AID - 33/5/1917 [pii]
PST - ppublish
SO  - Anticancer Res. 2013 May;33(5):1917-25.