PMID- 23645767
OWN - NLM
STAT- MEDLINE
DCOM- 20130626
LR  - 20191210
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 33
IP  - 5
DP  - 2013 May
TI  - Evaluation of current prognostic and predictive markers in breast cancer: a 
      validation study of tissue microarrays.
PG  - 2139-45
AB  - BACKGROUND: Tissue microarrays (TMAs) are an attractive alternative to analysis 
      of whole sections (WS). For breast carcinomas, the recent recommendations for 
      cut-offs (i.e. Ki67, H-score) have necessitated the re-evaluation of TMAs. 
      MATERIALS AND METHODS: TMA results of immunohistochemistry (IHC) and Fluorescence 
      in situ hybridization (FISH) testing for Estrogen receptors (ER), Progesterone 
      receptors (PgR), Ki67 and HER2 were compared against the results of WS for 88 
      breast carcinomas. RESULTS: We found excellent agreement between the two methods 
      for ER and PgR IHC evaluation, using the H-score (Kappa coefficient 0.972 and 
      0.9, respectively). There was also excellent correlation for HER2 IHC (Kappa 
      coefficient 1) and amplification (Kappa coefficient 0.933). Furthermore, scoring 
      of Ki67 was highly-correlated between TMAs and WS (Kappa coefficient 0.954). The 
      latter excellent correlation has not, to our knowledge, been previously reported. 
      CONCLUSION: For breast cancer, TMAs are an efficient and reliable alternative to 
      the use of WS, using the currently recommended markers, evaluation protocols and 
      cut-off values.
FAU - Batistatou, Anna
AU  - Batistatou A
AD  - Department of Pathology, University of Ioannina, Medical School, University 
      Campus, PO BOX 1186, 451 10, Ioannina, Greece. abatista@cc.uoi.gr
FAU - Televantou, Despina
AU  - Televantou D
FAU - Bobos, Mattheos
AU  - Bobos M
FAU - Eleftheraki, Anastasia G
AU  - Eleftheraki AG
FAU - Kouvaras, Evangelos
AU  - Kouvaras E
FAU - Chrisafi, Sofia
AU  - Chrisafi S
FAU - Koukoulis, George K
AU  - Koukoulis GK
FAU - Malamou-Mitsi, Vassiliki
AU  - Malamou-Mitsi V
FAU - Fountzilas, George
AU  - Fountzilas G
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Validation Study
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Breast Neoplasms/*diagnosis/genetics/metabolism
MH  - Carcinoma, Ductal, Breast/*diagnosis/genetics/metabolism
MH  - Carcinoma, Lobular/*diagnosis/genetics/metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Receptor, ErbB-2/genetics/metabolism
MH  - Receptors, Estrogen/genetics/metabolism
MH  - Receptors, Progesterone/genetics/metabolism
MH  - Tissue Array Analysis
OTO - NOTNLM
OT  - Breast cancer
OT  - ER
OT  - HER2
OT  - Ki67
OT  - PgR
OT  - TMA
EDAT- 2013/05/07 06:00
MHDA- 2013/06/28 06:00
CRDT- 2013/05/07 06:00
PHST- 2013/05/07 06:00 [entrez]
PHST- 2013/05/07 06:00 [pubmed]
PHST- 2013/06/28 06:00 [medline]
AID - 33/5/2139 [pii]
PST - ppublish
SO  - Anticancer Res. 2013 May;33(5):2139-45.