PMID- 23647163
OWN - NLM
STAT- MEDLINE
DCOM- 20140428
LR  - 20190806
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 75
IP  - 4
DP  - 2013 Aug
TI  - COP1 re-accumulates in the nucleus under shade.
PG  - 631-41
LID - 10.1111/tpj.12226 [doi]
AB  - Shade-avoider plants typically respond to shade-light signals by increasing the 
      rate of stem growth. CONSTITTUTIVE PHOTOMORPHOGENESIS 1 (COP1) is an E3 ligase 
      involved in the ubiquitin labelling of proteins targeted for degradation. In 
      dark-grown seedlings, COP1 accumulates in the nucleus and light exposure causes 
      COP1 migration to the cytosol. Here, we show that in Arabidopsis thaliana, COP1 
      accumulates in the nucleus under natural or simulated shade, despite the presence 
      of far-red light. In plants grown under white light, the transfer to shade-light 
      conditions triggers an unexpectedly rapid re-accumulation of COP1 in the nucleus. 
      The partial simulation of shade by lowering either blue or red light levels 
      (maintaining far-red light) caused COP1 nuclear re-accumulation. Hypocotyl growth 
      of wild-type seedlings is more sensitive to afternoon shade than to morning 
      shade. A residual response to shade was observed in the cop1 mutant background, 
      but these seedlings showed inverted sensitivity as they responded to morning 
      shade and not to afternoon shade. COP1 overexpression exaggerated the wild-type 
      pattern by enhancing afternoon sensitivity and making morning shade inhibitory of 
      growth. COP1 nuclear re-accumulation also responded more strongly to afternoon 
      shade than to morning shade. These results are consistent with a signalling role 
      of COP1 in shade avoidance. We propose a function of COP1 in setting the daily 
      patterns of sensitivity to shade in the fluctuating light environments of plant 
      canopies.
CI  - (c) 2013 The Authors The Plant Journal (c) 2013 John Wiley & Sons Ltd.
FAU - Pacin, Manuel
AU  - Pacin M
AD  - IFEVA, Facultad de Agronomia, Universidad de Buenos Aires and CONICET, 1417, 
      Buenos Aires, Argentina.
FAU - Legris, Martina
AU  - Legris M
FAU - Casal, Jorge J
AU  - Casal JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130530
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 2.3.2.27 (AT2G32950 protein, Arabidopsis)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Arabidopsis/cytology/genetics/*physiology/radiation effects
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Circadian Rhythm
MH  - *Gene Expression Regulation, Plant
MH  - Hypocotyl/cytology/genetics/physiology/radiation effects
MH  - *Light
MH  - Nuclear Proteins/genetics/metabolism
MH  - Recombinant Fusion Proteins
MH  - Seedlings/cytology/genetics/physiology/radiation effects
MH  - Signal Transduction
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
OTO - NOTNLM
OT  - Arabidopsis thaliana
OT  - CONSTITUTIVE PHOTOMORPHOGENESIS 1
OT  - growth
OT  - hypocotyl
OT  - shade avoidance
EDAT- 2013/05/08 06:00
MHDA- 2014/04/29 06:00
CRDT- 2013/05/08 06:00
PHST- 2013/01/13 00:00 [received]
PHST- 2013/04/25 00:00 [revised]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/05/08 06:00 [entrez]
PHST- 2013/05/08 06:00 [pubmed]
PHST- 2014/04/29 06:00 [medline]
AID - 10.1111/tpj.12226 [doi]
PST - ppublish
SO  - Plant J. 2013 Aug;75(4):631-41. doi: 10.1111/tpj.12226. Epub 2013 May 30.