PMID- 23647320
OWN - NLM
STAT- MEDLINE
DCOM- 20150403
LR  - 20191210
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 42
IP  - 2
DP  - 2013 Jun
TI  - Serum alpha1-proteinase inhibitor concentrations in healthy dogs--method 
      validation and determination of reference interval and intra-individual 
      variation.
PG  - 190-5
LID - 10.1111/vcp.12039 [doi]
AB  - BACKGROUND: A chronic loss of canine alpha1 -proteinase inhibitor (calpha1 -PI) into the 
      gastrointestinal (GI) tract could change the systemic proteinase-proteinase 
      inhibitor balance. Serum calpha1 -PI concentrations have not been studied in dogs 
      with well-defined GI diseases. OBJECTIVES: To further evaluate serum calpha1 -PI 
      concentrations in dogs with GI diseases, the objectives of this study were to (1) 
      analytically validate a previously developed fecal calpha1 -PI immunoassay to 
      determine serum concentrations, (2) determine a population-based reference 
      interval (RI) and assess the clinical utility, (3) determine stability of serum 
      calpha1 -PI, (4) determine the intra-individual variation in healthy dogs, and (5) 
      determine the clinically relevant magnitude of change of serum calpha1 -PI. METHODS: 
      Prestudy validation of the (125) I-calpha1 -PI immunoassay included linearity, 
      spiking recovery, and intra- and inter-assay precision. A RI was calculated with 
      samples of healthy dogs. Stability at -20 degrees C was tested on 36 samples. 
      Intra-individual variation was assessed using samples collected from 11 healthy 
      dogs over a 12-week period. RESULTS: The calpha1 -PI radioimmunoassay (RIA) was 
      linear, accurate, precise, and reproducible. Serum calpha1 -PI decreased by 11% after 
      one year at -20 degrees C. Analytical, intra-individual, inter-individual, and total 
      variation were 6.4, 9.9, 9.0, and 25.3%, respectively. The RI for serum calpha1 -PI 
      was 732-1802 mg/L (n = 87); there were no differences between sex and age groups. 
      The index of individuality was 1.31. As analytical variation was > (1/2) 
      inter-individual variation, the minimum critical difference was not determined. 
      CONCLUSIONS: The results of this study provide the basis for further evaluating 
      serum calpha1 -PI in dogs with GI disease. Using a population-based RI for serum calpha1 
      -PI appears to be appropriate.
CI  - (c) 2013 American Society for Veterinary Clinical Pathology.
FAU - Heilmann, Romy M
AU  - Heilmann RM
AD  - Department of Small Animal Clinical Sciences, College of Veterinary Medicine and 
      Biomedical Sciences, Gastrointestinal Laboratory, Texas A&M University, College 
      Station, TX 77843-4474, USA. rheilmann@cvm.tamu.edu
FAU - Ruaux, Craig G
AU  - Ruaux CG
FAU - Burgener, Iwan A
AU  - Burgener IA
FAU - Hern, Jennifer D
AU  - Hern JD
FAU - Suchodolski, Jan S
AU  - Suchodolski JS
FAU - Steiner, Jorg M
AU  - Steiner JM
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20130423
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Iodine Radioisotopes)
RN  - 0 (alpha 1-Antitrypsin)
SB  - IM
MH  - Animals
MH  - Dog Diseases/blood/enzymology
MH  - Dogs/*blood
MH  - Female
MH  - Gastrointestinal Diseases/blood/veterinary
MH  - Iodine Radioisotopes
MH  - Male
MH  - Radioimmunoassay/standards/*veterinary
MH  - Reference Values
MH  - Reproducibility of Results
MH  - alpha 1-Antitrypsin/*blood/standards
EDAT- 2013/05/08 06:00
MHDA- 2015/04/04 06:00
CRDT- 2013/05/08 06:00
PHST- 2013/05/08 06:00 [entrez]
PHST- 2013/05/08 06:00 [pubmed]
PHST- 2015/04/04 06:00 [medline]
AID - 10.1111/vcp.12039 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2013 Jun;42(2):190-5. doi: 10.1111/vcp.12039. Epub 2013 Apr 23.