PMID- 23648332
OWN - NLM
STAT- MEDLINE
DCOM- 20131209
LR  - 20130524
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 92
IP  - 5
DP  - 2013 Jul
TI  - Xenoestrogenic and dioxin-like activity in blood of East Greenland polar bears 
      (Ursus maritimus).
PG  - 583-91
LID - S0045-6535(13)00521-3 [pii]
LID - 10.1016/j.chemosphere.2013.03.059 [doi]
AB  - The aims of the project were to (i) extract the lipophilic persistent organic 
      pollutants (POPs) from the blood of 99 East Greenland polar bears and assess the 
      combined mixture effect on the estrogen receptor (ER) and the aryl hydrocarbon 
      receptor (AhR) mediated transactivity; (ii) To evaluate whether the receptor 
      transactivities were associated with selected POP markers, and (iii) compare the 
      receptor transactivities in polar bears with earlier studies on Greenlandic 
      Inuit. Lipophilic POPs were extracted using a combination of solid-phase 
      extraction (SPE) and high performance liquid chromatography (HPLC). ER mediated 
      transactivity was determined using the ER luciferase reporter MVLN cell assay. 
      The extracts were tested alone (XER) and together with 17beta-estradiol (E2) as a 
      physiological mimic (XERcomp). Dioxins and dioxin-like (DL) compounds were 
      extracted by a combination of SPE and the Supelco Dioxin Prep System(R). AhR 
      mediated dioxin-like transactivity was determined using the AhR luciferase 
      reporter Hepa 1.12cR cell assay. Agonistic ER transactivity was elicited by 19% 
      of the samples, and a further increased E2 induced ER response was found for 52%, 
      whereas 17% antagonized the E2 induced ER response. Positive correlations were 
      found in subadult bears between XER and several POP biomarkers. XER and XERcomp 
      correlated positively to each other. A total of 91% of the polar bear blood 
      extracts elicited agonistic AhR transactivity. The AhR-TCDD equivalent (AhR-TEQ) 
      median levels were higher among adult bears compared to subadult bears, but not 
      significantly.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Erdmann, Simon E
AU  - Erdmann SE
AD  - Centre for Arctic Health, Department of Public Health and Arctic Research Centre, 
      Aarhus University, Build. 1260, Bartholins Alle 2, 8000 Aarhus C, Denmark.
FAU - Dietz, Rune
AU  - Dietz R
FAU - Sonne, Christian
AU  - Sonne C
FAU - Bechshoft, Thea O
AU  - Bechshoft TO
FAU - Vorkamp, Katrin
AU  - Vorkamp K
FAU - Letcher, Robert J
AU  - Letcher RJ
FAU - Long, Manhai
AU  - Long M
FAU - Bonefeld-Jorgensen, Eva C
AU  - Bonefeld-Jorgensen EC
LA  - eng
PT  - Journal Article
DEP - 20130503
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Dioxins)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Pesticides)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Receptors, Estrogen)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Animals
MH  - Dioxins/*blood/metabolism
MH  - Environmental Pollutants/*blood/metabolism
MH  - Female
MH  - Male
MH  - Pesticides/blood/metabolism
MH  - Polychlorinated Biphenyls/blood/metabolism
MH  - Receptors, Aryl Hydrocarbon/*metabolism
MH  - Receptors, Estrogen/*metabolism
MH  - Ursidae/*blood/metabolism
EDAT- 2013/05/08 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/05/08 06:00
PHST- 2012/11/23 00:00 [received]
PHST- 2013/03/18 00:00 [revised]
PHST- 2013/03/22 00:00 [accepted]
PHST- 2013/05/08 06:00 [entrez]
PHST- 2013/05/08 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S0045-6535(13)00521-3 [pii]
AID - 10.1016/j.chemosphere.2013.03.059 [doi]
PST - ppublish
SO  - Chemosphere. 2013 Jul;92(5):583-91. doi: 10.1016/j.chemosphere.2013.03.059. Epub 
      2013 May 3.