PMID- 23649256
OWN - NLM
STAT- MEDLINE
DCOM- 20131231
LR  - 20130523
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 77
IP  - 5
DP  - 2013
TI  - Nitric oxide induces vascular endothelial growth factor expression in the rat 
      placenta in vivo and in vitro.
PG  - 971-6
AB  - We investigated the role of nitric oxide (NO) in vascular endothelial growth 
      factor (VEGF) expression in the rat placenta. A nitric oxide synthase (NOS) 
      inhibitor, N(G)-nitro-L-arginine-methyl ester (L-NAME), was constantly infused 
      into pregnant rats 6-24 h before sacrifice on gestational day (GD) 15.5. NO 
      production declined to about 15% of the control level as monitored by NO trapping 
      and electron paramagnetic resonance spectroscopy. VEGF mRNA expression was 
      temporally decreased by L-NAME, but recovered to normal levels after 24 h of 
      treatment, whereas hypoxia inducible factor (HIF)-1alpha and induced NOS (iNOS) 
      expression increased. VEGF expression decreased significantly in placental 
      explants after 6 h of co-treatment with L-NAME and lipopolysaccharide, an iNOS 
      inducer. Our data indicate that NO induce VEGF expression in vivo and in vitro in 
      the rat placenta, suggesting that peaked NO production was maintained by a 
      reciprocal relationship between NO and VEGF via HIF-1alpha.
FAU - Abe, Hideaki
AU  - Abe H
AD  - Graduate School of Veterinary Medicine, Azabu University, Fuchinobe, Chuo-ku, 
      Sagamihara, Japan.
FAU - Ishikawa, Wataru
AU  - Ishikawa W
FAU - Kushima, Takahiro
AU  - Kushima T
FAU - Nishimura, Tomoka
AU  - Nishimura T
FAU - Mori, Chiemi
AU  - Mori C
FAU - Onuki, Atsushi
AU  - Onuki A
FAU - Suzuki, Takehito
AU  - Suzuki T
FAU - Ishii, Yasuo
AU  - Ishii Y
FAU - Kansaku, Norio
AU  - Kansaku N
FAU - Miyazaki, Yoko
AU  - Miyazaki Y
FAU - Tanaka, Kazuaki
AU  - Tanaka K
FAU - Morita, Hidetoshi
AU  - Morita H
FAU - Takizawa, Tatsuya
AU  - Takizawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130507
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
RN  - V55S2QJN2X (NG-Nitroarginine Methyl Ester)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics
MH  - NG-Nitroarginine Methyl Ester/pharmacology
MH  - Nitric Oxide/*pharmacology
MH  - Nitric Oxide Synthase Type II/genetics
MH  - Placenta/*drug effects/*metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Vascular Endothelial Growth Factor A/*genetics
MH  - Vascular Endothelial Growth Factor Receptor-1/genetics
MH  - Vascular Endothelial Growth Factor Receptor-2/genetics
EDAT- 2013/05/08 06:00
MHDA- 2014/01/01 06:00
CRDT- 2013/05/08 06:00
PHST- 2013/05/08 06:00 [entrez]
PHST- 2013/05/08 06:00 [pubmed]
PHST- 2014/01/01 06:00 [medline]
AID - DN/JST.JSTAGE/bbb/120923 [pii]
AID - 10.1271/bbb.120923 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2013;77(5):971-6. doi: 10.1271/bbb.120923. Epub 2013 
      May 7.