PMID- 23650285
OWN - NLM
STAT- MEDLINE
DCOM- 20141023
LR  - 20181202
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 102
IP  - 4
DP  - 2014 Apr
TI  - Pore size-dependent immunogenic activity of mesoporous silica-based adjuvants in 
      cancer immunotherapy.
PG  - 967-74
LID - 10.1002/jbm.a.34783 [doi]
AB  - Commonly used aluminum hydroxide (Alum) adjuvant provokes a strong type 2 helper 
      T cell (Th2) response for mediating antibody production but is rather ineffective 
      for disease prevention that requires type 1 helper T cell (Th1) response for 
      mediating cellular immunity in human vaccination. Here, for the purpose of 
      inducing Th1 antitumor immunity, a mesoporous silica (MS)-based adjuvant is 
      prepared. Three kinds of MS particles with nearly identical particle size and 
      surface area but different pore sizes of 4, 7 and 10 nm were prepared. No serious 
      in vitro cytotoxicity was observed for the MS particles at 5, 20, 50, and 100 
      mug/mL. Pathogen-associated molecular patterns (PAMPs) were immobilized with 
      apatite (Ap) on MS to prepare the MS-based and PAMP-loaded adjuvants (MS-Ap-PAMP 
      adjuvants). Macrophage-like cells cultured in the presence of MS-Ap-PAMP adjuvant 
      with a MS pore size of 10 nm showed the maximum in vitro immunogenic activity. 
      Injection of the MS-Ap-PAMP adjuvant with a MS pore size of 10 nm in combination 
      with liquid nitrogen-treated tumor tissue (derived from Lewis lung carcinoma 
      cells) to C57BL/6 mice markedly inhibited the development of rechallenged tumor 
      in vivo, while no such antitumor immunity was induced in injection of Alum mixed 
      with PAMP in combination with liquid nitrogen-treated tumor tissue. The 
      MS-Ap-PAMP adjuvant contributed to the elicitation of a potent systemic Th1 
      antitumor immunity in vivo.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Wang, Xiupeng
AU  - Wang X
AD  - Human Technology Research Institute, National Institute of Advanced Industrial 
      Science and Technology (AIST), Tsukuba, Ibaraki, 305-8566, Japan.
FAU - Li, Xia
AU  - Li X
FAU - Ito, Atsuo
AU  - Ito A
FAU - Sogo, Yu
AU  - Sogo Y
FAU - Ohno, Tadao
AU  - Ohno T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130528
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Apatites)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Adjuvants, Immunologic/pharmacology/*therapeutic use
MH  - Adsorption
MH  - Animals
MH  - Apatites/chemistry
MH  - Carcinoma, Lewis Lung/drug therapy/immunology
MH  - Cell Survival/drug effects
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - *Immunotherapy
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron, Scanning
MH  - NIH 3T3 Cells
MH  - Neoplasms/*drug therapy/*immunology
MH  - Nitrogen
MH  - Porosity
MH  - Silicon Dioxide/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - adjuvant
OT  - immune
OT  - mesoporous silica
OT  - pore size
EDAT- 2013/05/08 06:00
MHDA- 2014/10/24 06:00
CRDT- 2013/05/08 06:00
PHST- 2013/04/01 00:00 [received]
PHST- 2013/04/29 00:00 [revised]
PHST- 2013/04/29 00:00 [accepted]
PHST- 2013/05/08 06:00 [entrez]
PHST- 2013/05/08 06:00 [pubmed]
PHST- 2014/10/24 06:00 [medline]
AID - 10.1002/jbm.a.34783 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2014 Apr;102(4):967-74. doi: 10.1002/jbm.a.34783. Epub 2013 
      May 28.