PMID- 23651585
OWN - NLM
STAT- MEDLINE
DCOM- 20140506
LR  - 20160518
IS  - 1876-7753 (Electronic)
IS  - 1873-5061 (Linking)
VI  - 11
IP  - 1
DP  - 2013 Jul
TI  - Long-term repopulating hematopoietic stem cells and "side population" in human 
      steady state peripheral blood.
PG  - 625-33
LID - S1873-5061(13)00041-X [pii]
LID - 10.1016/j.scr.2013.04.003 [doi]
AB  - This report brings the first experimental evidence for the presence of long-term 
      (LT) repopulating hematopoietic stem cells (HSCs) and Side Population (SP) cells 
      within human steady state peripheral blood CD34(+) cells. Ex vivo culture, which 
      reveals the LT-HSC, also increases short-term (ST) HSC engraftment capacity and 
      SP cell number (as well as the SP subpopulations defined on the basis of CD38, 
      CD90 and CD133 expression) which are very low in freshly isolated cells. Thus, ex 
      vivo incubation either allows the expansion of the small fraction of HSCs or 
      reveals "Scid Repopulating Cells - SRC" that are present in the initial CD34(+) 
      cell population but unable to engraft. In addition, among these CD34(+) cells, we 
      confirm the presence of committed progenitors at frequencies similar to those 
      found in cord blood CD34(+) cells. These cells, obtained from leukoreduction 
      filters (LRFs) and rejected in the course of the preparation of red blood cell 
      concentrates, are an abundant and reliable material for obtaining committed 
      progenitors, short- and long-term HSCs of therapeutic interest, especially after 
      the ex vivo expansion phase. Our results open a perspective to set up new 
      therapeutic protocols using expanded LRFs-recovered CD34(+) cells as a source of 
      HSCs for autologous or allogeneic transplantation.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Brunet de la Grange, Philippe
AU  - Brunet de la Grange P
AD  - Etablissement Francais du Sang Aquitaine-Limousin, 33035 Bordeaux, France.
FAU - Vlaski, Marija
AU  - Vlaski M
FAU - Duchez, Pascale
AU  - Duchez P
FAU - Chevaleyre, Jean
AU  - Chevaleyre J
FAU - Lapostolle, Veronique
AU  - Lapostolle V
FAU - Boiron, Jean-Michel
AU  - Boiron JM
FAU - Praloran, Vincent
AU  - Praloran V
FAU - Ivanovic, Zoran
AU  - Ivanovic Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130411
PL  - England
TA  - Stem Cell Res
JT  - Stem cell research
JID - 101316957
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Hematopoietic Stem Cells/*cytology/immunology/metabolism
MH  - Humans
MH  - Mice
MH  - Mice, Inbred NOD
MH  - Mice, SCID
MH  - Side-Population Cells/*cytology/immunology/metabolism
EDAT- 2013/05/09 06:00
MHDA- 2014/05/07 06:00
CRDT- 2013/05/09 06:00
PHST- 2013/03/26 00:00 [received]
PHST- 2013/04/03 00:00 [accepted]
PHST- 2013/05/09 06:00 [entrez]
PHST- 2013/05/09 06:00 [pubmed]
PHST- 2014/05/07 06:00 [medline]
AID - S1873-5061(13)00041-X [pii]
AID - 10.1016/j.scr.2013.04.003 [doi]
PST - ppublish
SO  - Stem Cell Res. 2013 Jul;11(1):625-33. doi: 10.1016/j.scr.2013.04.003. Epub 2013 
      Apr 11.