PMID- 23651687 OWN - NLM STAT- MEDLINE DCOM- 20140912 LR - 20211203 IS - 1743-1328 (Electronic) IS - 0161-6412 (Linking) VI - 35 IP - 8 DP - 2013 Oct TI - Effects of luteolin on spatial memory, cell proliferation, and neuroblast differentiation in the hippocampal dentate gyrus in a scopolamine-induced amnesia model. PG - 813-20 LID - 10.1179/1743132813Y.0000000217 [doi] AB - OBJECTIVES: Luteolin, a common flavonoid from many plants, has various pharmacological activities, including a memory-improving effect. In this study, we investigated the effects of luteolin on spatial memory, cell proliferation, and neuroblast differentiation in the hippocampal dentate gyrus in a rat model of scopolamine (SCO)-induced amnesia. METHODS: Scopolamine was subcutaneously administered for 28 days via an Alzet minipump (44 mg/ml delivered at 2.5 mul/h) along with a daily intraperitoneal administration of vehicle (saline) 10 mg/kg luteolin or 5 mg/kg galantamine (GAL) (a control drug for acetylcholinesterase (AChE) inhibitor) for 28 days. RESULTS: The administration of SCO significantly decreased the spatial alteration percentage in the Y-maze test compared to that in the vehicle (saline)-treated group. The administration of luteolin or GAL significantly improved the spatial alteration percentage compared to that in the SCO-treated group. Similarly, the administration of SCO significantly decreased the cell proliferation (Ki67-positive cells) and neuroblast differentiation (doubleocortin-positive cells) in the dentate gyrus. The administration of luteolin or GAL significantly mitigated the SCO-induced reduction of Ki67- and doublecortin-immunoreactive cells in the dentate gyrus. In addition, the administration of luteolin significantly decreased the lipid peroxidation (malondialdehyde (MDA) levels) and increased the brain-derived neurotrophic factor (BDNF) and AChE levels in the hippocampal homogenates compared to the SCO-treated group. CONCLUSION: These results suggest that the luteolin treatment improves the SCO-induced reduction of cell proliferation and neuroblast differentiation in the dentate gyrus. The mechanism underlying the amelioration of SCO-induced amnesia by luteolin may be associated with the increase in BDNF, acetylcholine, and the decrease in lipid peroxidation. FAU - Yoo, Dae Young AU - Yoo DY AD - Seoul National University, Seoul, South Korea. FAU - Choi, Jung Hoon AU - Choi JH FAU - Kim, Woosuk AU - Kim W FAU - Nam, Sung Min AU - Nam SM FAU - Jung, Hyo Young AU - Jung HY FAU - Kim, Jong Hwi AU - Kim JH FAU - Won, Moo-Ho AU - Won MH FAU - Yoon, Yeo Sung AU - Yoon YS FAU - Hwang, In Koo AU - Hwang IK LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130503 PL - England TA - Neurol Res JT - Neurological research JID - 7905298 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Cholinesterase Inhibitors) RN - 0 (Dcx protein, rat) RN - 0 (Doublecortin Protein) RN - 0 (Neuroprotective Agents) RN - 0D3Q044KCA (Galantamine) RN - DL48G20X8X (Scopolamine) RN - EC 3.1.1.7 (Acetylcholinesterase) RN - KUX1ZNC9J2 (Luteolin) SB - IM MH - Acetylcholinesterase/metabolism MH - Amnesia/chemically induced/drug therapy/*pathology MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cell Differentiation/*drug effects MH - Cell Proliferation/*drug effects MH - Cholinesterase Inhibitors/pharmacology MH - Dentate Gyrus/cytology/*drug effects/pathology/physiopathology MH - Doublecortin Protein MH - Galantamine/pharmacology MH - Lipid Peroxidation/drug effects MH - Luteolin/*pharmacology/therapeutic use MH - Male MH - Maze Learning MH - Memory Disorders/chemically induced/*drug therapy MH - Neurogenesis/drug effects MH - Neuroprotective Agents/*pharmacology/therapeutic use MH - Rats MH - Scopolamine EDAT- 2013/05/09 06:00 MHDA- 2014/09/13 06:00 CRDT- 2013/05/09 06:00 PHST- 2013/05/09 06:00 [entrez] PHST- 2013/05/09 06:00 [pubmed] PHST- 2014/09/13 06:00 [medline] AID - ner3187 [pii] AID - 10.1179/1743132813Y.0000000217 [doi] PST - ppublish SO - Neurol Res. 2013 Oct;35(8):813-20. doi: 10.1179/1743132813Y.0000000217. Epub 2013 May 3.