PMID- 23652995
OWN - NLM
STAT- MEDLINE
DCOM- 20140129
LR  - 20211021
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 40
IP  - 7
DP  - 2013 Jul
TI  - Heterogeneity of 11q13 region rearrangements in laryngeal squamous cell carcinoma 
      analyzed by microarray platforms and fluorescence in situ hybridization.
PG  - 4161-71
LID - 10.1007/s11033-013-2496-4 [doi]
AB  - We reinvestigated rearrangements occurring in region q13 of chromosome 11 aiming 
      to: (i) describe heterogeneity of the observed structural alterations, (ii) 
      estimate amplicon size and (iii) identify of oncogenes involved in laryngeal 
      cancer progression as potential targets for therapy. The study included 17 cell 
      lines derived from laryngeal cancers and 34 specimens from primary laryngeal 
      tumors. The region 11q13 was analyzed by fluorescence in situ hybridization 
      (FISH), array comparative genomic hybridization (aCGH) and gene expression 
      microarray. Next, quantitative real time PCR was used for chosen genes to confirm 
      results from aCGH and gene expression microarray. The observed pattern of 
      aberrations allows to distinguish three ways, in which gain and amplification 
      involving 11q13 region may occur: formation of a homogeneously staining region; 
      breakpoints in/near 11q13, which lead to the three to sevenfold increase of the 
      copy number of 11q13 region; the presence of additional copies of the whole 
      chromosome 11. The minimal altered region of gain and/or amplification was 
      limited to ~1.8 Mb (chr.11:69,395,184-71,209,568) and comprised mostly 11q13.3 
      band which contain 12 genes. Five, out of these genes (CCND1, ORAOV1, FADD, 
      PPFIA1, CTTN) had higher expression levels in comparison to healthy controls. 
      Apart from CCND1 gene, which has an established role in pathogenesis of head and 
      neck cancers, CTTN, ORAOV1 and FADD genes appear to be oncogene-candidates in 
      laryngeal cancers, while a function of PPFIA1 requires further studies.
FAU - Jarmuz-Szymczak, Malgorzata
AU  - Jarmuz-Szymczak M
AD  - Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479, 
      Poznan, Poland. maljar@man.poznan.pl
FAU - Pelinska, Kinga
AU  - Pelinska K
FAU - Kostrzewska-Poczekaj, Magdalena
AU  - Kostrzewska-Poczekaj M
FAU - Bembnista, Ewa
AU  - Bembnista E
FAU - Giefing, Maciej
AU  - Giefing M
FAU - Brauze, Damian
AU  - Brauze D
FAU - Szaumkessel, Marcin
AU  - Szaumkessel M
FAU - Marszalek, Andrzej
AU  - Marszalek A
FAU - Janiszewska, Joanna
AU  - Janiszewska J
FAU - Kiwerska, Katarzyna
AU  - Kiwerska K
FAU - Bartochowska, Anna
AU  - Bartochowska A
FAU - Grenman, Reidar
AU  - Grenman R
FAU - Szyfter, Witold
AU  - Szyfter W
FAU - Szyfter, Krzysztof
AU  - Szyfter K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130508
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - *Chromosomes, Human, Pair 11
MH  - Cluster Analysis
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - Gene Dosage
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
EDAT- 2013/05/09 06:00
MHDA- 2014/01/30 06:00
CRDT- 2013/05/09 06:00
PHST- 2013/02/16 00:00 [received]
PHST- 2013/04/24 00:00 [accepted]
PHST- 2013/05/09 06:00 [entrez]
PHST- 2013/05/09 06:00 [pubmed]
PHST- 2014/01/30 06:00 [medline]
AID - 10.1007/s11033-013-2496-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2013 Jul;40(7):4161-71. doi: 10.1007/s11033-013-2496-4. Epub 2013 
      May 8.