PMID- 23653154
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130509
LR  - 20220809
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 2
IP  - 5
DP  - 2013 May 7
TI  - Vectofusin-1, a new viral entry enhancer, strongly promotes lentiviral 
      transduction of human hematopoietic stem cells.
PG  - e90
LID - 10.1038/mtna.2013.17 [doi]
AB  - Gene transfer into hCD34(+) hematopoietic stem/progenitor cells (HSCs) using 
      human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors (LVs) has 
      several promising therapeutic applications. Yet, efficiency, safety, and cost of 
      LV gene therapy could be ameliorated by enhancing target cell transduction levels 
      and reducing the amount of LV used on the cells. Several transduction enhancers 
      already exist such as fibronectin fragments and cationic compounds, but all 
      present limitations. In this study, we describe a new transduction enhancer 
      called Vectofusin-1, which is a short cationic peptide, active on several LV 
      pseudotypes. Vectofusin-1 is used as a soluble additive to safely increase the 
      frequency of transduced HSCs and to augment the level of transduction to one or 
      two copies of vector per cell in a vector dose-dependent manner. Vectofusin-1 
      acts at the entry step by promoting the adhesion and the fusion between viral and 
      cellular membranes. Vectofusin-1 is therefore a promising additive that could 
      significantly ameliorate hCD34(+) cell-based gene therapy.Molecular 
      Therapy-Nucleic Acids (2013) 2, e90; doi:10.1038/mtna.2013.17; published online 7 
      May 2013.
FAU - Fenard, David
AU  - Fenard D
AD  - 1] Genethon, Evry, France [2] INSERM, UMR_S951, Genethon, Evry, France [3] 
      Universite Evry Val d'Essonne, UMR_S951, Evry, France.
FAU - Ingrao, Dina
AU  - Ingrao D
FAU - Seye, Ababacar
AU  - Seye A
FAU - Buisset, Julien
AU  - Buisset J
FAU - Genries, Sandrine
AU  - Genries S
FAU - Martin, Samia
AU  - Martin S
FAU - Kichler, Antoine
AU  - Kichler A
FAU - Galy, Anne
AU  - Galy A
LA  - eng
PT  - Journal Article
DEP - 20130507
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC4817938
EDAT- 2013/05/09 06:00
MHDA- 2013/05/09 06:01
PMCR- 2013/05/01
CRDT- 2013/05/09 06:00
PHST- 2013/05/09 06:00 [entrez]
PHST- 2013/05/09 06:00 [pubmed]
PHST- 2013/05/09 06:01 [medline]
PHST- 2013/05/01 00:00 [pmc-release]
AID - S2162-2531(16)30149-4 [pii]
AID - 10.1038/mtna.2013.17 [doi]
PST - epublish
SO  - Mol Ther Nucleic Acids. 2013 May 7;2(5):e90. doi: 10.1038/mtna.2013.17.