PMID- 23653160
OWN - NLM
STAT- MEDLINE
DCOM- 20131021
LR  - 20211021
IS  - 1932-8494 (Electronic)
IS  - 1932-8486 (Print)
IS  - 1932-8486 (Linking)
VI  - 296
IP  - 7
DP  - 2013 Jul
TI  - Analysis of the effect of estrogen/androgen perturbation on penile development in 
      transgenic and diethylstilbestrol-treated mice.
PG  - 1127-41
LID - 10.1002/ar.22708 [doi]
AB  - Because both androgens and estrogens have been implicated in penile 
      morphogenesis, we evaluated penile morphology in transgenic mice with known 
      imbalance of androgen and estrogen signaling using scanning electron microscopy 
      (SEM), histology, and immunohistochemistry of androgen and estrogen receptors 
      alpha/beta. Penises of adult wild-type, estrogen receptor-alpha knockout (alphaERKO), estrogen 
      receptor-beta knockout (betaERKO), aromatase knockout (Arom-KO), and aromatase 
      overexpression (Arom+) mice were evaluated, as well as adult mice treated with 
      diethylstilbestrol (DES) from birth to day 10. Adult penises were examined 
      because the adult pattern is the endpoint of development. The urethral orifice is 
      formed by fusion of the MUMP (male urogenital mating protuberance) with the MUMP 
      ridge, which consists of several processes fused to each other and to the MUMP. 
      Similarly, the internal prepuce is completed ventrally by fusion of a ventral 
      cleft. In adult murine penises the stromal processes that form the MUMP ridge are 
      separated from their neighbors by clefts. alphaERKO, betaERKO, and Arom-KO mice have 
      penises with a MUMP ridge clefting pattern similar to that of wild-type mice. In 
      contrast, Arom+ mice and neonatally DES-treated mice exhibit profound 
      malformations of the MUMP, MUMP ridge clefting pattern, and internal prepuce. 
      Abnormalities observed in Arom+ and neonatally DES-treated mice correlate with 
      the expression of estrogen receptor-beta (ERbeta) in the affected structures. This 
      study demonstrates that formation of the urethal orifice and internal prepuce is 
      due to fusion of separate epithelial-surfaced mesenchymal elements, a process 
      dependent upon both androgen and estrogen signaling, in which ERbeta signaling is 
      strongly implicated.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Blaschko, Sarah D
AU  - Blaschko SD
AD  - Department of Urology, University of California San Francisco, A610 San 
      Francisco, California, USA.
FAU - Mahawong, Phitsanu
AU  - Mahawong P
FAU - Ferretti, Max
AU  - Ferretti M
FAU - Cunha, Tristan J
AU  - Cunha TJ
FAU - Sinclair, Adriane
AU  - Sinclair A
FAU - Wang, Hong
AU  - Wang H
FAU - Schlomer, Bruce J
AU  - Schlomer BJ
FAU - Risbridger, Gail
AU  - Risbridger G
FAU - Baskin, Laurence S
AU  - Baskin LS
FAU - Cunha, Gerald R
AU  - Cunha GR
LA  - eng
GR  - R01 DK058105/DK/NIDDK NIH HHS/United States
GR  - R01 DK0581050/DK/NIDDK NIH HHS/United States
GR  - APP1002733/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130508
PL  - United States
TA  - Anat Rec (Hoboken)
JT  - Anatomical record (Hoboken, N.J. : 2007)
JID - 101292775
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens, Non-Steroidal)
RN  - 731DCA35BT (Diethylstilbestrol)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Aromatase/deficiency/genetics/*metabolism
MH  - Diethylstilbestrol/*toxicity
MH  - Estrogen Receptor alpha/deficiency/genetics/*metabolism
MH  - Estrogen Receptor beta/deficiency/genetics/*metabolism
MH  - Estrogens, Non-Steroidal/*toxicity
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Microscopy, Electron, Scanning
MH  - Morphogenesis/drug effects
MH  - Penis/abnormalities/*drug effects/metabolism
MH  - Signal Transduction/drug effects
PMC - PMC3709018
MID - NIHMS491246
EDAT- 2013/05/09 06:00
MHDA- 2013/10/22 06:00
PMCR- 2014/07/01
CRDT- 2013/05/09 06:00
PHST- 2013/02/06 00:00 [received]
PHST- 2013/03/27 00:00 [accepted]
PHST- 2013/05/09 06:00 [entrez]
PHST- 2013/05/09 06:00 [pubmed]
PHST- 2013/10/22 06:00 [medline]
PHST- 2014/07/01 00:00 [pmc-release]
AID - 10.1002/ar.22708 [doi]
PST - ppublish
SO  - Anat Rec (Hoboken). 2013 Jul;296(7):1127-41. doi: 10.1002/ar.22708. Epub 2013 May 
      8.