PMID- 23653849
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130509
LR  - 20211021
IS  - 2008-3866 (Print)
IS  - 2008-3874 (Electronic)
IS  - 2008-3866 (Linking)
VI  - 15
IP  - 6
DP  - 2012 Nov
TI  - In vitro Assay of Human Gingival Scaffold in Differentiation of Rat's Bone Marrow 
      Mesenchymal Stem Cells to Keratinocystes.
PG  - 1185-90
AB  - OBJECTIVES: Tissue engineering is an attractive science because it promises new 
      therapeutic strategies for repairing organs that have lost functions due to 
      damage. The purpose of this study was to evaluate induction effect of human 
      gingival scaffold in tissue engineering for skin regeneration. MATERIALS AND 
      METHODS: Tissue samples were obtained from crown-lengthening procedures and 
      wisdom teeth removal. The samples were decellularized and used as a scaffold for 
      loading of rat BM-MSCs. The human gingival scaffolds loaded by bone marrow 
      mesenchymal stem cells were derived from Wistar rat. Finally, it was evaluated 
      via electron micrographs, as well as immunohistochemical techniques at day 7, 14, 
      and 28 after initial seeding. RESULTS: The histologic sections of human gingival 
      scaffold -loaded rat BM-MSCs demonstrated formation of epithelial like layers at 
      days 7, 14 and 28 after initial seeding. Scanning electron microscope (SEM) of 
      the scaffolds indicated formed desmosomal adhesions, which revealed a degree of 
      differentiation toward keratinocytes. The results of immunohistochemical staining 
      were strongly positive for multi cytokeratin (CK) 14 days after initial seeding 
      in epithelial differentiation. Rat BM-MSCs which loaded on human gingival 
      scaffold is capable of differentiating toward keratinocytes. CONCLUSION: Gingival 
      tissues were presented as a natural scaffold for attachment and differentiation 
      of bone marrow mesenchymal stem cells towards keratinocytes, and might be used as 
      suitable scaffold for reconstruction of the skin.
FAU - Mahdavishahri, Nasser
AU  - Mahdavishahri N
AD  - Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, 
      Mashhad, Iran.
FAU - Moghatam Matin, Maryam
AU  - Moghatam Matin M
FAU - Fereidoni, Masoud
AU  - Fereidoni M
FAU - Yarjanli, Zahra
AU  - Yarjanli Z
FAU - Banihashem Rad, Seyed Ali
AU  - Banihashem Rad SA
FAU - Khajeh Ahmadi, Saeedeh
AU  - Khajeh Ahmadi S
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Basic Med Sci
JT  - Iranian journal of basic medical sciences
JID - 101517966
PMC - PMC3646230
OTO - NOTNLM
OT  - Acellular gingiva
OT  - Keratinocytes
OT  - Tissue engineering
EDAT- 2013/05/09 06:00
MHDA- 2013/05/09 06:01
PMCR- 2012/11/01
CRDT- 2013/05/09 06:00
PHST- 2011/11/02 00:00 [received]
PHST- 2012/02/25 00:00 [accepted]
PHST- 2013/05/09 06:00 [entrez]
PHST- 2013/05/09 06:00 [pubmed]
PHST- 2013/05/09 06:01 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - IJBMS-15-1185 [pii]
PST - ppublish
SO  - Iran J Basic Med Sci. 2012 Nov;15(6):1185-90.