PMID- 23656318
OWN - NLM
STAT- MEDLINE
DCOM- 20140106
LR  - 20130617
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 30
IP  - 5
DP  - 2013
TI  - Multiplex immunoassay for persistent organic pollutants in tilapia: comparison of 
      imaging- and flow cytometry-based platforms using spectrally encoded paramagnetic 
      microspheres.
PG  - 843-52
LID - 10.1080/19440049.2013.769138 [doi]
AB  - Recent developments in spectrally encoded microspheres (SEMs)-based technologies 
      provide high multiplexing possibilities. Most SEMs-based assays require a flow 
      cytometer with sophisticated fluidics and optics. A new imaging 
      super-paramagnetic SEMs-based alternative platform transports SEMs with 
      considerably less fluid volume into a measuring chamber. Once there SEMs are held 
      in a monolayer by a magnet. Light-emitting diodes (LEDs) are focused on the 
      chamber to illuminate the SEMs - instead of lasers and they are imaged by a 
      charge-coupled device (CCD) detector, offering a more compact sized, 
      transportable and affordable system. The feasibility of utilising this system to 
      develop a 3-plex SEMs-based imaging immunoassay (IMIA) for the screening of 
      persistent organic pollutants (POPs) was studied. Moreover the performance 
      characteristics of 3-plex IMIA were critically compared with the conventional 
      3-plex flow cytometric immunoassay (FCIA). Both SEM technologies have potential 
      for the multiplex analysis of polychlorinated biphenyls (PCBs), polybrominated 
      diphenyl ethers (PBDEs) and polycyclic aromatic hydrocarbons (PAHs) in buffer and 
      fish extract with insignificant differences in assay sensitivities. Furthermore, 
      we developed a faster and simpler, modified QuEChERS-like generic POPs extraction 
      from tilapia fillet using sodium hydrogen carbonate as one of the salt additives 
      and dispersive solid-phase extraction (dSPE) as a clean-up. Finally, a 
      preliminary in-house validation using 40 different blank and spiked tilapia 
      fillet samples was performed in both systems and the results obtained were 
      critically compared. The lower-cost imaging SEMs-based system performed similarly 
      to the original flow cytometer and, in combination with the new quicker 
      QuEChERS-like extraction, it has high potential for future rapid screening of 
      POPs in several other sample matrices such as other fish species, vegetable 
      refined oils and environmental samples.
FAU - Meimaridou, Anastasia
AU  - Meimaridou A
AD  - RIKILT-Institute of Food Safety, Wageningen University and Research Center, 
      Wageningen, The Netherlands.
FAU - Haasnoot, Willem
AU  - Haasnoot W
FAU - Shelver, Weilin L
AU  - Shelver WL
FAU - Franek, Milan
AU  - Franek M
FAU - Nielen, Michel W F
AU  - Nielen MW
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130508
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Organic Chemicals)
RN  - 0 (Water Pollutants, Chemical)
SB  - IM
MH  - Animals
MH  - Feasibility Studies
MH  - Flow Cytometry/*methods
MH  - Immunoassay/*methods
MH  - *Microspheres
MH  - Organic Chemicals/*analysis
MH  - *Tilapia
MH  - Water Pollutants, Chemical/*analysis
EDAT- 2013/05/10 06:00
MHDA- 2014/01/07 06:00
CRDT- 2013/05/10 06:00
PHST- 2013/05/10 06:00 [entrez]
PHST- 2013/05/10 06:00 [pubmed]
PHST- 2014/01/07 06:00 [medline]
AID - 10.1080/19440049.2013.769138 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(5):843-52. 
      doi: 10.1080/19440049.2013.769138. Epub 2013 May 8.