PMID- 23657866 OWN - NLM STAT- MEDLINE DCOM- 20160108 LR - 20161125 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 102 IP - 1 DP - 2014 Jan TI - Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture. PG - 247-53 LID - 10.1002/jbma.34695 [doi] AB - The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water-holding capability. These results suggest that the Dextran-coated PVF sponges with high water-holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial. CI - Copyright (c) 2013 Wiley Periodicals, Inc. FAU - Togami, Wakana AU - Togami W AD - Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860-8556, Japan. FAU - Sei, Akira AU - Sei A FAU - Okada, Tatsuya AU - Okada T FAU - Taniwaki, Takuya AU - Taniwaki T FAU - Fujimoto, Toru AU - Fujimoto T FAU - Nakamura, Takayuki AU - Nakamura T FAU - Tahata, Shogo AU - Tahata S FAU - Nakanishi, Yoshitaka AU - Nakanishi Y FAU - Mizuta, Hiroshi AU - Mizuta H LA - eng PT - Journal Article DEP - 20130509 PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 RN - 0 (Antigens, Differentiation) RN - 0 (Coated Materials, Biocompatible) RN - 0 (Dextrans) RN - 059QF0KO0R (Water) SB - IM MH - Animals MH - Antigens, Differentiation/biosynthesis MH - Bone Marrow Cells/cytology/*metabolism MH - Cells, Cultured MH - Coated Materials, Biocompatible/*chemistry MH - Dextrans/*chemistry MH - Male MH - *Osteogenesis MH - Rats MH - Rats, Inbred F344 MH - Stem Cells/cytology/*metabolism MH - Water/*chemistry OTO - NOTNLM OT - bone marrow stem cells OT - coated polyvinyl formal sponge OT - high water-holding capability OT - osteogenic ability EDAT- 2013/05/10 06:00 MHDA- 2016/01/09 06:00 CRDT- 2013/05/10 06:00 PHST- 2013/02/24 00:00 [received] PHST- 2013/03/01 00:00 [accepted] PHST- 2013/05/10 06:00 [entrez] PHST- 2013/05/10 06:00 [pubmed] PHST- 2016/01/09 06:00 [medline] AID - 10.1002/jbma.34695 [doi] PST - ppublish SO - J Biomed Mater Res A. 2014 Jan;102(1):247-53. doi: 10.1002/jbma.34695. Epub 2013 May 9.