PMID- 23657870
OWN - NLM
STAT- MEDLINE
DCOM- 20140121
LR  - 20181203
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 31
IP  - 5
DP  - 2013 Jul
TI  - Gender depended potentiality of differentiation of human umbilical cord 
      mesenchymal stem cells into oocyte-Like cells in vitro.
PG  - 365-73
LID - 10.1002/cbf.2981 [doi]
AB  - Recent studies have demonstrated that germ-like cells could be differentiated 
      from human umbilical cord mesenchymal stem cells (hUC-MSCs) in vitro. Whether the 
      sexuality of hUC-MSCs affects the formation efficiency of germ-like cells derived 
      from hUC-MSCs is still unclear. To clearly test the formation efficiency of 
      oocyte-like cells from male and female hUC-MSCs, obtained hUC-MSCs were induced 
      by 20% follicular fluid (FF) according to the method that has been proved by our 
      previous studies. Results showed that hUC-MSCs differentiated into oocyte-like 
      structures and expressed germ cell makers. It was noted that the presence of 
      advanced oocyte-like cells in male hUC-MSCs (m-hUC-MSCs) was similar as that in 
      female hUC-MSCs (f-hUC-MSCs); however, the expression of germ cell's specific 
      markers in m-hUC-MSCs was delayed compared with that in f-hUC-MSCs. In addition, 
      immunofluorescence analysis demonstrated that germ cell-specific markers, Oct4, 
      Vasa, Dazl, ZP2, ZP3 and Stra8, were expressed on the 14th day after induction in 
      both f-hUC-MSCs and m-hUC-MSCs. However, the size of oocyte-like cells from 
      f-hUC-MSCs was larger than that in m-hUC-MSCs. The level of secreted oestradiol 
      was significantly higher in f-hUC-MSCs than m-hUC-MSCs. We sought to determine 
      whether critical germ cell's transcription factor-Figlalpha will promote the 
      development of oocyte-like cells. Some germ cell-specific markers were increased 
      when exogenous Figlalpha was transfected into hUC-MSCs. This process implied that 
      germ-like cells might be produced by over-expression of exogenous germ 
      cell-specific gene, and this process was similar as that in production of germ 
      cells in induced pluripotent stem cells (iPSCs). Finally, to verify the 
      feasibility that hUC-MSCs differentiate into germ cells, hUC-MSCs were 
      transplanted into seminiferous tubules and kidney capsule of mouse, respectively, 
      and we found the transplanted cells differentiated into germ-like cells in 
      recipient's seminiferous tubules and kidney capsule. This study will provide a 
      simple model to study mammalian germ cell specification using hUC-MSCs in vitro.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - Qiu, Pubin
AU  - Qiu P
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & 
      Technology, Key Lab for Animal Biotechnology of Agriculture Ministry of China, 
      Northwest A&F University, Yangling, Shaanxi, China.
FAU - Bai, Yaofu
AU  - Bai Y
FAU - Pan, Shaohui
AU  - Pan S
FAU - Li, Wei
AU  - Li W
FAU - Liu, Weishuai
AU  - Liu W
FAU - Hua, Jinlian
AU  - Hua J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130509
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Biomarkers)
RN  - 0 (Figla protein, mouse)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Size
MH  - Cells, Cultured
MH  - Estradiol/metabolism
MH  - Female
MH  - Fetal Blood/*cytology/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Kidney/cytology/metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Oocytes/*cytology/metabolism
MH  - Seminiferous Tubules/cytology/metabolism
MH  - Sex Factors
MH  - Transfection
MH  - Transplantation, Heterologous
OTO - NOTNLM
OT  - Figlalpha
OT  - germ cells
OT  - mesenchymal stem cells (MSCs)
OT  - umbilical cord (UC)
EDAT- 2013/05/10 06:00
MHDA- 2014/01/22 06:00
CRDT- 2013/05/10 06:00
PHST- 2013/01/28 00:00 [received]
PHST- 2013/04/05 00:00 [revised]
PHST- 2013/04/09 00:00 [accepted]
PHST- 2013/05/10 06:00 [entrez]
PHST- 2013/05/10 06:00 [pubmed]
PHST- 2014/01/22 06:00 [medline]
AID - 10.1002/cbf.2981 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2013 Jul;31(5):365-73. doi: 10.1002/cbf.2981. Epub 2013 May 
      9.