PMID- 23657958
OWN - NLM
STAT- MEDLINE
DCOM- 20131028
LR  - 20191210
IS  - 1097-0231 (Electronic)
IS  - 0951-4198 (Linking)
VI  - 25
IP  - 18
DP  - 2011 Sep 30
TI  - A metabonomic analysis of urine from rats treated with rhizoma alismatis using 
      ultra-performance liquid chromatography/mass spectrometry.
PG  - 2633-40
LID - 10.1002/rcm.5163 [doi]
AB  - A metabonomic approach based on ultra-performance liquid chromatography coupled 
      to mass spectrometry (UPLC/MS) was used to study the nephrotoxicity of rhizoma 
      alismatis (RA) in rats. Potential biomarkers of RA toxicity were identified and 
      the toxicological mechanism is discussed. Urine samples were collected from 
      control and treated rats at various stages and analyzed by UPLC/MS in positive 
      ionization mode. Histopathological analysis was used to evaluate renal function. 
      The differences in the metabolic profiles of the control and treated rats were 
      clearly distinguishable with principal components analysis (PCA) of the 
      chromatographic data, and significant changes in 13 metabolite biomarkers were 
      detected in the urine. This metabonomic method combined with PCA could 
      discriminate the treated rats from the control rats on days 60, 120, and 180 
      after treatment, before serious organic renal damage was apparent on day 180 with 
      histopathology. This research indicates that UPLC/MS-based metabonomic analysis 
      of urine samples can be used to predict the chronic nephrotoxicity induced by 
      rhizoma alismatis.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Yu, Yue
AU  - Yu Y
AD  - Department of Pharmaceutical Analysis, Shenyang Pharmaceutical University, Wenhua 
      Road 103, Shenyang, 110016, PR China.
FAU - Ma, Chao
AU  - Ma C
FAU - Bi, Kaishun
AU  - Bi K
FAU - Yang, Guoguang
AU  - Yang G
FAU - Xie, Pu
AU  - Xie P
FAU - Wang, Jie
AU  - Wang J
FAU - Chen, Xiao-Hui
AU  - Chen XH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Drugs, Chinese Herbal)
SB  - IM
MH  - Alisma/*chemistry
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Disease Models, Animal
MH  - Drugs, Chinese Herbal/*analysis/metabolism/toxicity
MH  - Humans
MH  - Kidney/drug effects/metabolism
MH  - Kidney Diseases/*chemically induced/metabolism/urine
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Metabolomics/*methods
MH  - Rats
MH  - Rats, Wistar
MH  - Rhizome/adverse effects/*chemistry
MH  - Urine/*chemistry
EDAT- 2011/09/30 00:00
MHDA- 2013/10/29 06:00
CRDT- 2013/05/10 06:00
PHST- 2011/03/02 00:00 [received]
PHST- 2011/06/21 00:00 [revised]
PHST- 2011/06/27 00:00 [accepted]
PHST- 2013/05/10 06:00 [entrez]
PHST- 2011/09/30 00:00 [pubmed]
PHST- 2013/10/29 06:00 [medline]
AID - 10.1002/rcm.5163 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2011 Sep 30;25(18):2633-40. doi: 10.1002/rcm.5163.