PMID- 23659350
OWN - NLM
STAT- MEDLINE
DCOM- 20131023
LR  - 20211021
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Print)
IS  - 1936-0851 (Linking)
VI  - 7
IP  - 6
DP  - 2013 Jun 25
TI  - Tiny grains give huge gains: nanocrystal-based signal amplification for 
      biomolecule detection.
PG  - 5142-50
LID - 10.1021/nn400733t [doi]
AB  - Nanocrystals, despite their tiny sizes, contain thousands to millions of atoms. 
      Here we show that the large number of atoms packed in each metallic nanocrystal 
      can provide a huge gain in signal amplification for biomolecule detection. We 
      have devised a highly sensitive, linear amplification scheme by integrating the 
      dissolution of bound nanocrystals and metal-induced stoichiometric chromogenesis, 
      and demonstrated that signal amplification is fully defined by the size and atom 
      density of nanocrystals, which can be optimized through well-controlled 
      nanocrystal synthesis. Further, the rich library of chromogenic reactions allows 
      implementation of this scheme in various assay formats, as demonstrated by the 
      iron oxide nanoparticle linked immunosorbent assay (ILISA) and blotting assay 
      developed in this study. Our results indicate that, owing to the inherent 
      simplicity, high sensitivity and repeatability, the nanocrystal based 
      amplification scheme can significantly improve biomolecule quantification in both 
      laboratory research and clinical diagnostics. This novel method adds a new 
      dimension to current nanoparticle-based bioassays.
FAU - Tong, Sheng
AU  - Tong S
AD  - Department of Biomedical Engineering, Georgia Institute of Technology and Emory 
      University, Atlanta, Georgia 30332, USA.
FAU - Ren, Binbin
AU  - Ren B
FAU - Zheng, Zhilan
AU  - Zheng Z
FAU - Shen, Han
AU  - Shen H
FAU - Bao, Gang
AU  - Bao G
LA  - eng
GR  - HHSN268201000043C/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130513
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Ferric Compounds)
RN  - 0 (Ferrous Compounds)
RN  - 1K09F3G675 (ferric oxide)
RN  - G7036X8B5H (ferrous oxide)
SB  - IM
MH  - Biological Assay/*methods
MH  - Blotting, Western
MH  - Ferric Compounds/chemistry
MH  - Ferrous Compounds/chemistry
MH  - Immunosorbent Techniques
MH  - Kinetics
MH  - Models, Molecular
MH  - Molecular Conformation
MH  - Nanoparticles/*chemistry
PMC - PMC3758924
MID - NIHMS480648
EDAT- 2013/05/11 06:00
MHDA- 2013/10/24 06:00
PMCR- 2014/06/25
CRDT- 2013/05/11 06:00
PHST- 2013/05/11 06:00 [entrez]
PHST- 2013/05/11 06:00 [pubmed]
PHST- 2013/10/24 06:00 [medline]
PHST- 2014/06/25 00:00 [pmc-release]
AID - 10.1021/nn400733t [doi]
PST - ppublish
SO  - ACS Nano. 2013 Jun 25;7(6):5142-50. doi: 10.1021/nn400733t. Epub 2013 May 13.