PMID- 23660532
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20220223
IS  - 1432-0436 (Electronic)
IS  - 0301-4681 (Print)
IS  - 0301-4681 (Linking)
VI  - 86
IP  - 3
DP  - 2013 Oct
TI  - Extracellular matrix proteins regulate epithelial-mesenchymal transition in 
      mammary epithelial cells.
PG  - 126-32
LID - S0301-4681(13)00025-X [pii]
LID - 10.1016/j.diff.2013.03.003 [doi]
AB  - Mouse mammary epithelial cells undergo transdifferentiation via 
      epithelial-mesenchymal transition (EMT) upon treatment with matrix 
      metalloproteinase-3 (MMP3). In rigid microenvironments, MMP3 upregulates 
      expression of Rac1b, which translocates to the cell membrane to promote induction 
      of reactive oxygen species and EMT. Here we examine the role of the extracellular 
      matrix (ECM) in this process. Our data show that the basement membrane protein 
      laminin suppresses the EMT response in MMP3-treated cells, whereas fibronectin 
      promotes EMT. These ECM proteins regulate EMT via interactions with their 
      specific integrin receptors. alpha6-integrin sequesters Rac1b from the membrane and 
      is required for inhibition of EMT by laminin. In contrast, alpha5-integrin maintains 
      Rac1b at the membrane and is required for the promotion of EMT by fibronectin. 
      Understanding the regulatory role of the ECM will provide insight into mechanisms 
      underlying normal and pathological development of the mammary gland.
CI  - (c) 2013 International Society of Differentiation. Published by Elsevier B.V. All 
      rights reserved.
FAU - Chen, Qike K
AU  - Chen QK
AD  - Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, 
      USA.
FAU - Lee, KangAe
AU  - Lee K
FAU - Radisky, Derek C
AU  - Radisky DC
FAU - Nelson, Celeste M
AU  - Nelson CM
LA  - eng
GR  - R21 HL110335/HL/NHLBI NIH HHS/United States
GR  - HL110335/HL/NHLBI NIH HHS/United States
GR  - R01 GM083997/GM/NIGMS NIH HHS/United States
GR  - CA128660/CA/NCI NIH HHS/United States
GR  - R21 CA128660/CA/NCI NIH HHS/United States
GR  - GM083997/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130506
PL  - England
TA  - Differentiation
JT  - Differentiation; research in biological diversity
JID - 0401650
RN  - 0 (Fibronectins)
RN  - 0 (Integrin alpha5)
RN  - 0 (Integrin alpha6)
RN  - 0 (Laminin)
RN  - 0 (Neuropeptides)
RN  - 0 (Rac1 protein, mouse)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
RN  - EC 3.6.5.2 (rac1 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Epithelial Cells/*drug effects/metabolism/physiology
MH  - Epithelial-Mesenchymal Transition/*drug effects
MH  - Extracellular Space/metabolism
MH  - Female
MH  - Fibronectins/*pharmacology
MH  - Integrin alpha5/genetics/metabolism
MH  - Integrin alpha6/genetics/metabolism
MH  - Laminin/*pharmacology
MH  - Mammary Glands, Animal/*cytology
MH  - Matrix Metalloproteinase 3/*pharmacology
MH  - Mice
MH  - Neuropeptides/genetics/metabolism
MH  - Stress, Mechanical
MH  - rac1 GTP-Binding Protein/genetics/metabolism
PMC - PMC3762919
MID - NIHMS469160
OTO - NOTNLM
OT  - Alpha-smooth muscle actin
OT  - BM
OT  - Basement membrane
OT  - Cell shape
OT  - ECM
OT  - EMT
OT  - EMyT
OT  - Epithelial-mesenchymal transition
OT  - Epithelial-myofibroblast transition
OT  - Extracellular matrix
OT  - Integrin
OT  - Laminin-rich ECM
OT  - MMP3
OT  - Matrix metalloproteinase-3
OT  - Mechanical stress
OT  - ROS
OT  - Reactive oxygen species
OT  - TGFbeta
OT  - Transforming growth factor-beta
OT  - lrECM
OT  - alphaSMA
EDAT- 2013/05/11 06:00
MHDA- 2014/05/03 06:00
PMCR- 2014/10/01
CRDT- 2013/05/11 06:00
PHST- 2012/12/20 00:00 [received]
PHST- 2013/03/20 00:00 [accepted]
PHST- 2013/05/11 06:00 [entrez]
PHST- 2013/05/11 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - S0301-4681(13)00025-X [pii]
AID - 10.1016/j.diff.2013.03.003 [doi]
PST - ppublish
SO  - Differentiation. 2013 Oct;86(3):126-32. doi: 10.1016/j.diff.2013.03.003. Epub 
      2013 May 6.