PMID- 23662405
OWN - NLM
STAT- MEDLINE
DCOM- 20140123
LR  - 20130513
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 29
IP  - 1
DP  - 2013 Jan
TI  - [The role of TLR4-mediated MyD88-dependent pathway in neuroinflammation in 
      hippocampal neurons of rats].
PG  - 42-6
AB  - OBJECTIVE: To investigate weather there is a toll-like receptor 4 (TLR4)-mediated 
      myeloid differentiation factor 88 (MyD88)-dependent pathway in hippocampal 
      neurons of rats and the probable role of the pathway in neuroinflammation. 
      METHODS: To establish the proper model, primarily cultured hippocampal neurons 
      were treated with lipopolysaccharides (LPS), or pretreated with TLR4 antibody 
      then co-treated with LPS. The expression of mRNA of MyD88 and TNF-alpha receptor 
      associated factor 6 (TRAF6) were tested by RT-qPCR. The content of MyD88 and 
      TRAF6 were tested by Western blot. The nuclear translocation of nuclear 
      factor-kappaB/P65 (NF-kappaB/p65) was tested by immunofluorescence. The content 
      of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and 
      nitric oxide (NO) were tested by ELISA. RESULTS: LPS could increase MyD88 and 
      TRAF6 mRNA, upregulate protein level of MyD88 and TRAF6 and increase the level of 
      TNF-alpha, IL-1beta and NO in cell culture supernatant. LPS also could promote 
      NF-kappa B/p65 translation to the nucleus. The pretreatment with TLR4 antibody 
      reduced the translocation to nucleus for NF-kappaB/P65 and the contents of 
      TNF-alpha, IL-1beta and NO in the culture supernatant. CONCLUSION: There is a 
      TLR4-mediated MyD88-dependent pathway in hippocampal neurons. The activation of 
      this pathway can increase the level of TNF-alpha, IL-1beta and NO in cell culture 
      supernatant. TLR4-mediated MyD88-dependent pathway in hippocampal neurons 
      participate in neuroinflammation, that means neurons are not passive in 
      inflammation.
FAU - Zhang, Guo-Xia
AU  - Zhang GX
AD  - Department of Pathophysiology, Medical College of Nantong University, Nantong 
      226001, China.
FAU - Zhou, Ai-Ling
AU  - Zhou AL
FAU - Zhang, Gui-Ping
AU  - Zhang GP
FAU - Hu, Ya-E
AU  - Hu YE
FAU - Mao, Jia-Hui
AU  - Mao JH
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Interleukin-1beta)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Rela protein, rat)
RN  - 0 (TNF Receptor-Associated Factor 6)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Hippocampus/*cytology/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Myeloid Differentiation Factor 88/*metabolism
MH  - Neuritis/*metabolism
MH  - Neurons/*metabolism
MH  - Nitric Oxide/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - TNF Receptor-Associated Factor 6/metabolism
MH  - Toll-Like Receptor 4/*metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2013/05/15 06:00
MHDA- 2014/01/24 06:00
CRDT- 2013/05/14 06:00
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/01/24 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2013 Jan;29(1):42-6.