PMID- 23663187
OWN - NLM
STAT- MEDLINE
DCOM- 20140226
LR  - 20181202
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 115
IP  - 2
DP  - 2013 Aug
TI  - Correlation analysis of Shiga toxin-producing Escherichia coli shedding and 
      faecal bacterial composition in beef cattle.
PG  - 591-603
LID - 10.1111/jam.12250 [doi]
AB  - AIMS: The objectives of this study were to investigate the correlations between 
      Shiga toxin-producing Escherichia coli (STEC) shedding and faecal microflora in 
      beef cattle and to identify functional species that might be used for STEC 
      control. METHODS AND RESULTS: Faecal samples were collected from 110 calves and 
      92 dams. The number and prevalence of STEC were determined using CHROMagar STEC; 
      denaturing gradient gel electrophoresis (DGGE) was employed to analyse faecal 
      bacterial composition. Six-month-old calves had the highest STEC shedding levels 
      (3.03 +/- 1.41 Log CFU g(-1)) and prevalence (95.5%). Both the number and 
      prevalence decreased significantly as the calf age increased (P < 0.05). The DGGE 
      analysis showed that faecal bacterial diversity increased, while cattle ages 
      increased and STEC shedding levels decreased. Significant correlations between 
      STEC shedding, cattle age and bacterial compositions were observed by redundancy 
      analysis (P < 0.05). T-value biplots and sequencing results indicated that 
      butyrate-producing bacteria (BPB) negatively correlated with STEC shedding. 
      CONCLUSIONS: Higher STEC shedding levels and prevalence were associated with 
      younger cattle age, lower faecal bacterial diversity and lower BPB levels. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: Butyrate-producing bacteria in GI tract 
      might serve as an option for the future development of STEC shedding control 
      strategy.
CI  - (c) 2013 The Society for Applied Microbiology.
FAU - Zhao, L
AU  - Zhao L
AD  - Food Microbiology and Safety Lab, Department of Animal Sciences, Auburn 
      University, Auburn, AL 36849, USA.
FAU - Tyler, P J
AU  - Tyler PJ
FAU - Starnes, J
AU  - Starnes J
FAU - Bratcher, C L
AU  - Bratcher CL
FAU - Rankins, D
AU  - Rankins D
FAU - McCaskey, T A
AU  - McCaskey TA
FAU - Wang, L
AU  - Wang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130530
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - *Bacterial Shedding
MH  - Cattle/*microbiology
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Feces/*microbiology
MH  - Female
MH  - Meat
MH  - Shiga-Toxigenic Escherichia coli/genetics/*isolation & purification
OTO - NOTNLM
OT  - Shiga toxin-producing Escherichia coli
OT  - age
OT  - butyrate-producing bacteria
OT  - calf
OT  - dam
OT  - faecal microflora
EDAT- 2013/05/15 06:00
MHDA- 2014/02/27 06:00
CRDT- 2013/05/14 06:00
PHST- 2013/03/06 00:00 [received]
PHST- 2013/05/06 00:00 [revised]
PHST- 2013/05/08 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2014/02/27 06:00 [medline]
AID - 10.1111/jam.12250 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2013 Aug;115(2):591-603. doi: 10.1111/jam.12250. Epub 2013 May 
      30.