PMID- 23663784
OWN - NLM
STAT- MEDLINE
DCOM- 20130716
LR  - 20161125
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 153
IP  - 4
DP  - 2013 May 9
TI  - Roquin promotes constitutive mRNA decay via a conserved class of stem-loop 
      recognition motifs.
PG  - 869-81
LID - S0092-8674(13)00457-1 [pii]
LID - 10.1016/j.cell.2013.04.016 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is the most potent proinflammatory cytokine in 
      mammals. The degradation of TNF-alpha mRNA is critical for restricting TNF-alpha 
      synthesis and involves a constitutive decay element (CDE) in the 3' UTR of the 
      mRNA. Here, we demonstrate that the CDE folds into an RNA stem-loop motif that is 
      specifically recognized by Roquin and Roquin2. Binding of Roquin initiates 
      degradation of TNF-alpha mRNA and limits TNF-alpha production in macrophages. Roquin 
      proteins promote mRNA degradation by recruiting the Ccr4-Caf1-Not deadenylase 
      complex. CDE sequences are highly conserved and are found in more than 50 
      vertebrate mRNAs, many of which encode regulators of development and 
      inflammation. In macrophages, CDE-containing mRNAs were identified as the primary 
      targets of Roquin on a transcriptome-wide scale. Thus, Roquin proteins act 
      broadly as mediators of mRNA deadenylation by recognizing a conserved class of 
      stem-loop RNA degradation motifs.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Leppek, Kathrin
AU  - Leppek K
AD  - Helmholtz Junior Research Group Posttranscriptional Control of Gene Expression, 
      German Cancer Research Center, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 280, 69120 
      Heidelberg, Germany.
FAU - Schott, Johanna
AU  - Schott J
FAU - Reitter, Sonja
AU  - Reitter S
FAU - Poetz, Fabian
AU  - Poetz F
FAU - Hammond, Ming C
AU  - Hammond MC
FAU - Stoecklin, Georg
AU  - Stoecklin G
LA  - eng
SI  - GEO/GSE44775
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (3' Untranslated Regions)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (roquin-2 protein, mouse)
RN  - EC 2.3.2.27 (Rc3h1 protein, mouse)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Humans
MH  - Inflammation/metabolism
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Nucleotide Motifs
MH  - *RNA Stability
MH  - RNA, Messenger/chemistry
MH  - Repressor Proteins/*metabolism
MH  - Sequence Alignment
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Ubiquitin-Protein Ligases/*metabolism
EDAT- 2013/05/15 06:00
MHDA- 2013/07/17 06:00
CRDT- 2013/05/14 06:00
PHST- 2012/01/14 00:00 [received]
PHST- 2012/12/07 00:00 [revised]
PHST- 2013/04/05 00:00 [accepted]
PHST- 2013/05/14 06:00 [entrez]
PHST- 2013/05/15 06:00 [pubmed]
PHST- 2013/07/17 06:00 [medline]
AID - S0092-8674(13)00457-1 [pii]
AID - 10.1016/j.cell.2013.04.016 [doi]
PST - ppublish
SO  - Cell. 2013 May 9;153(4):869-81. doi: 10.1016/j.cell.2013.04.016.